Characterization of ordered aggregates of cerato-platanin and their involvement in fungus-host interactions

Luigia Pazzagli, Camilla Zoppi, Lara Carresi, Bruno Tiribilli, Francesca Sbrana, Silvia Schiff, Thelma A. Pertinhez, Aniello Scala, Gianni Cappugi

Research output: Contribution to journalArticle

Abstract

Background: The "cerato-platanin family" consists of fungal-secreted proteins that are involved in various stages of the host-fungus interaction and act as phytotoxins, elicitors of defense responses and allergens. Cerato-platanin (CP) is a moderately hydrophobic protein secreted and localized in the cell wall of Ceratocystis platani, the causal agent of a severe disease of Platanus. These properties make CP like the hydrophobins: these are self-assembling proteins that form a surface coating which is involved in the formation of aerial hyphae and in adherence to surfaces. Methods: CP aggregation was monitored by ThT, circular dichroism, and AFM. The eliciting activity of CP aggregates was assayed on leaves and cells. Results: The CP self-assembles forming amyloid-like aggregates via a nucleated growth mechanism which is joined up with a cleavage of the N-terminus. The ovoidal shape and the lack of a clear transition toward an all-β structure distinguish these aggregates from typical amyloid fibrils. Moreover, CP aggregates interact with hydrophobic surfaces and enhance the hypersensitive response of Platanus. Conclusion and general significance: CP forms "ordered aggregates" for which the soluble prefibrillar structures are the end point of the aggregation process, and do not evolve to insoluble fibrils. An involvement in host-microbe interaction is also suggested.

Original languageEnglish
Pages (from-to)1334-1344
Number of pages11
JournalBBA - General Subjects
Volume1790
Issue number10
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Amyloid-like assemblage
  • Cerato-platanin family
  • Fungal protein
  • Host-microbe interaction
  • Hydrophobin
  • Protein aggregation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Pazzagli, L., Zoppi, C., Carresi, L., Tiribilli, B., Sbrana, F., Schiff, S., Pertinhez, T. A., Scala, A., & Cappugi, G. (2009). Characterization of ordered aggregates of cerato-platanin and their involvement in fungus-host interactions. BBA - General Subjects, 1790(10), 1334-1344. https://doi.org/10.1016/j.bbagen.2009.07.014