Abstract
Objectives: VIM-type enzymes are among the most widespread acquired metallo-β-lactamases among Gramnegative pathogens. Integron In70 is a class 1 integron that has emerged as a successful genetic support for blaVIM-1 (one of themostprevalent blaVIM allelic variants) in Gram-negativenon-fermenters, andis usually chromosome borne. The objective of this study was to characterize plasmid pAX22 from Achromobacter xylosoxidans AX22, which represents the only In70-harbouring plasmid known so far, to gather insights into the mechanisms of evolution and dissemination of In70-like elements. Methods: The complete sequence of pAX22was obtained by pyrosequencing and assembled with Roche Newbler software. The draft sequence, completed using a PCR-based strategy, was annotated via the BASys tool and compared with known sequences using BLAST algorithms. Results: The backbone of pAX22 showed significant similarity with that of pNOR-2000, a blaVIM-2-harbouring plasmid from Pseudomonas aeruginosa, and with the TnCP23 transposon. The three elements differed from each other mainly by the class 1 integron cassette arrays and by some integron-associated structures. In pAX22, In70was associated with a novel putative transposon, Tn7017, composed of a defective Tn402-like transposon carrying In70 and the ISPa17 insertion sequence. Conclusion: Plasmid pAX22 belongs to a lineage of plasmids circulating among Gram-negative non-fermenters. In70 was probably acquired by pAX22 by transposition of Tn7017, revealing a novel putative mechanism of In70 mobilization. Our results highlight the potential role that ISPa17couldhave in mobilizing defective Tn402-like transposons carrying class 1 integrons.
| Original language | English |
|---|---|
| Article number | dkt311 |
| Pages (from-to) | 67-71 |
| Number of pages | 5 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 69 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2014 |
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Keywords
- Gram negative
- Insertion sequence
- Transposon
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)
- Infectious Diseases
Cite this
Characterization of plasmid pAX22, encoding VIM-1 metallo-β-lactamase, reveals a new putative mechanism of In70 integron mobilization. / Pilato, Vincenzo Di; Pollini, Simona; Rossolini, Gian Maria.
In: Journal of Antimicrobial Chemotherapy, Vol. 69, No. 1, dkt311, 01.2014, p. 67-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of plasmid pAX22, encoding VIM-1 metallo-β-lactamase, reveals a new putative mechanism of In70 integron mobilization
AU - Pilato, Vincenzo Di
AU - Pollini, Simona
AU - Rossolini, Gian Maria
PY - 2014/1
Y1 - 2014/1
N2 - Objectives: VIM-type enzymes are among the most widespread acquired metallo-β-lactamases among Gramnegative pathogens. Integron In70 is a class 1 integron that has emerged as a successful genetic support for blaVIM-1 (one of themostprevalent blaVIM allelic variants) in Gram-negativenon-fermenters, andis usually chromosome borne. The objective of this study was to characterize plasmid pAX22 from Achromobacter xylosoxidans AX22, which represents the only In70-harbouring plasmid known so far, to gather insights into the mechanisms of evolution and dissemination of In70-like elements. Methods: The complete sequence of pAX22was obtained by pyrosequencing and assembled with Roche Newbler software. The draft sequence, completed using a PCR-based strategy, was annotated via the BASys tool and compared with known sequences using BLAST algorithms. Results: The backbone of pAX22 showed significant similarity with that of pNOR-2000, a blaVIM-2-harbouring plasmid from Pseudomonas aeruginosa, and with the TnCP23 transposon. The three elements differed from each other mainly by the class 1 integron cassette arrays and by some integron-associated structures. In pAX22, In70was associated with a novel putative transposon, Tn7017, composed of a defective Tn402-like transposon carrying In70 and the ISPa17 insertion sequence. Conclusion: Plasmid pAX22 belongs to a lineage of plasmids circulating among Gram-negative non-fermenters. In70 was probably acquired by pAX22 by transposition of Tn7017, revealing a novel putative mechanism of In70 mobilization. Our results highlight the potential role that ISPa17couldhave in mobilizing defective Tn402-like transposons carrying class 1 integrons.
AB - Objectives: VIM-type enzymes are among the most widespread acquired metallo-β-lactamases among Gramnegative pathogens. Integron In70 is a class 1 integron that has emerged as a successful genetic support for blaVIM-1 (one of themostprevalent blaVIM allelic variants) in Gram-negativenon-fermenters, andis usually chromosome borne. The objective of this study was to characterize plasmid pAX22 from Achromobacter xylosoxidans AX22, which represents the only In70-harbouring plasmid known so far, to gather insights into the mechanisms of evolution and dissemination of In70-like elements. Methods: The complete sequence of pAX22was obtained by pyrosequencing and assembled with Roche Newbler software. The draft sequence, completed using a PCR-based strategy, was annotated via the BASys tool and compared with known sequences using BLAST algorithms. Results: The backbone of pAX22 showed significant similarity with that of pNOR-2000, a blaVIM-2-harbouring plasmid from Pseudomonas aeruginosa, and with the TnCP23 transposon. The three elements differed from each other mainly by the class 1 integron cassette arrays and by some integron-associated structures. In pAX22, In70was associated with a novel putative transposon, Tn7017, composed of a defective Tn402-like transposon carrying In70 and the ISPa17 insertion sequence. Conclusion: Plasmid pAX22 belongs to a lineage of plasmids circulating among Gram-negative non-fermenters. In70 was probably acquired by pAX22 by transposition of Tn7017, revealing a novel putative mechanism of In70 mobilization. Our results highlight the potential role that ISPa17couldhave in mobilizing defective Tn402-like transposons carrying class 1 integrons.
KW - Gram negative
KW - Insertion sequence
KW - Transposon
UR - http://www.scopus.com/inward/record.url?scp=84890343488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890343488&partnerID=8YFLogxK
U2 - 10.1093/jac/dkt311
DO - 10.1093/jac/dkt311
M3 - Article
VL - 69
SP - 67
EP - 71
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 1
M1 - dkt311
ER -