Characterization of red cell membrane proteins as a function of red cell density: . Annexin VII in different forms of hereditary spherocytosis

Marianna Caterino, Margherita Ruoppolo, Stefania Orrù, Marcella Savoia, Silverio Perrotta, Luigi Del Vecchio, Francesco Salvatore, Gordon W. Stewart, Achille Iolascon

Research output: Contribution to journalArticlepeer-review

Abstract

Fresh human blood samples were collected from healthy controls and splenectomized and unsplenectomized patients with hereditary spherocytosis due to band 3 or ankyrin and spectrin deficiency. The erythrocytes were separated into age-related fractions using self-forming Percoll density gradients. Membrane proteins were analysed by 2D electrophoresis and identified by mass spectrometry. Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation.

Original languageEnglish
Pages (from-to)6527-6532
Number of pages6
JournalFEBS Letters
Volume580
Issue number28-29
DOIs
Publication statusPublished - Dec 11 2006

Keywords

  • Annexin VII
  • Hereditary spherocytosis
  • Mass spectrometry
  • Membrane proteins
  • Proteomics
  • Red blood cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Characterization of red cell membrane proteins as a function of red cell density: . Annexin VII in different forms of hereditary spherocytosis'. Together they form a unique fingerprint.

Cite this