Characterization of red cell membrane proteins as a function of red cell density: . Annexin VII in different forms of hereditary spherocytosis

Marianna Caterino, Margherita Ruoppolo, Stefania Orrù, Marcella Savoia, Silverio Perrotta, Luigi Del Vecchio, Francesco Salvatore, Gordon W. Stewart, Achille Iolascon

Research output: Contribution to journalArticle

Abstract

Fresh human blood samples were collected from healthy controls and splenectomized and unsplenectomized patients with hereditary spherocytosis due to band 3 or ankyrin and spectrin deficiency. The erythrocytes were separated into age-related fractions using self-forming Percoll density gradients. Membrane proteins were analysed by 2D electrophoresis and identified by mass spectrometry. Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation.

Original languageEnglish
Pages (from-to)6527-6532
Number of pages6
JournalFEBS Letters
Volume580
Issue number28-29
DOIs
Publication statusPublished - Dec 11 2006

Keywords

  • Annexin VII
  • Hereditary spherocytosis
  • Mass spectrometry
  • Membrane proteins
  • Proteomics
  • Red blood cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Caterino, M., Ruoppolo, M., Orrù, S., Savoia, M., Perrotta, S., Vecchio, L. D., Salvatore, F., Stewart, G. W., & Iolascon, A. (2006). Characterization of red cell membrane proteins as a function of red cell density: . Annexin VII in different forms of hereditary spherocytosis. FEBS Letters, 580(28-29), 6527-6532. https://doi.org/10.1016/j.febslet.2006.10.070