Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury

Maria Lucia Angelotti, Elisa Ronconi, Lara Ballerini, Anna Peired, Benedetta Mazzinghi, Costanza Sagrinati, Eliana Parente, Mauro Gacci, Marco Carini, Mario Rotondi, Agnes B. Fogo, Elena Lazzeri, Laura Lasagni, Paola Romagnani

Research output: Contribution to journalArticlepeer-review

Abstract

Recent studies implicated the existence in adult human kidney of a population of renal progenitors with the potential to regenerate glomerular as well as tubular epithelial cells and characterized by coexpression of surface markers CD133 and CD24. Here, we demonstrate that CD133+CD24+ renal progenitors can be distinguished in distinct subpopulations from normal human kidneys based on the surface expression of vascular cell adhesion molecule 1, also known as CD106. CD133+CD24+CD106+ cells were localized at the urinary pole of Bowman's capsule, while a distinct population of scattered CD133+CD24+CD106- cells was localized in the proximal tubule as well as in the distal convoluted tubule. CD133+CD24+CD106+ cells exhibited a high proliferative rate and could differentiate toward the podocyte as well as the tubular lineage. By contrast, CD133+CD24+CD106- cells showed a lower proliferative capacity and displayed a committed phenotype toward the tubular lineage. Both CD133+CD24+CD106+ and CD133+CD24+CD106- cells showed higher resistance to injurious agents in comparison to all other differentiated cells of the kidney. Once injected in SCID mice affected by acute tubular injury, both of these populations displayed the capacity to engraft within the kidney, generate novel tubular cells, and improve renal function. These properties were not shared by other tubular cells of the adult kidney. Finally, CD133+CD24+CD106- cells proliferated upon tubular injury, becoming the predominating part of the regenerating epithelium in patients with acute or chronic tubular damage. These data suggest that CD133+CD24+CD106- cells represent tubular-committed progenitors that display resistance to apoptotic stimuli and exert regenerative potential for injured tubular tissue.

Original languageEnglish
Pages (from-to)1714-1725
Number of pages12
JournalStem Cells
Volume30
Issue number8
DOIs
Publication statusPublished - Aug 2012

Keywords

  • Acute kidney injury
  • Kidney
  • Renal progenitors
  • Renal stem cells
  • Tubular progenitor

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

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