Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

G. Gerna; Antonella Sarasini; Maria Torsellini; Angela di Matteo; F. Baldanti; M. Parea; M. Battaglia

doi:10.1007/BF01317927

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

G. Gerna, Antonella Sarasini, Maria Torsellini, Angela di Matteo, F. Baldanti, M. Parea, M. Battaglia

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article

Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

Original language	English
Pages (from-to)	315-322
Number of pages	8
Journal	Archives of Virology
Volume	107
Issue number	3-4
DOIs	https://doi.org/10.1007/BF01317927
Publication status	Published - Sep 1989

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ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Genetics

Cite this

Gerna, G., Sarasini, A., Torsellini, M., di Matteo, A., Baldanti, F., Parea, M., & Battaglia, M. (1989). Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains. Archives of Virology, 107(3-4), 315-322. https://doi.org/10.1007/BF01317927

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains. / Gerna, G.; Sarasini, Antonella; Torsellini, Maria; di Matteo, Angela ; Baldanti, F.; Parea, M.; Battaglia, M.

In: Archives of Virology, Vol. 107, No. 3-4, 09.1989, p. 315-322.

Research output: Contribution to journal › Article

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10.1007/BF01317927