Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

G. Gerna; Antonella Sarasini; Maria Torsellini; Angela di Matteo; F. Baldanti; M. Parea; M. Battaglia

doi:10.1007/BF01317927

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

G. Gerna, Antonella Sarasini, Maria Torsellini, Angela di Matteo, F. Baldanti, M. Parea, M. Battaglia

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

Original language	English
Pages (from-to)	315-322
Number of pages	8
Journal	Archives of Virology
Volume	107
Issue number	3-4
DOIs	https://doi.org/10.1007/BF01317927
Publication status	Published - Sep 1989

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Genetics

Access to Document

10.1007/BF01317927

Cite this

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains. / Gerna, G.; Sarasini, Antonella; Torsellini, Maria; di Matteo, Angela ; Baldanti, F.; Parea, M.; Battaglia, M.

In: Archives of Virology, Vol. 107, No. 3-4, 09.1989, p. 315-322.

Research output: Contribution to journal › Article › peer-review

@article{a121b9f4ab244b75a65bbb36a52e744e,

title = "Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains",

abstract = "Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.",

author = "G. Gerna and Antonella Sarasini and Maria Torsellini and {di Matteo}, Angela and F. Baldanti and M. Parea and M. Battaglia",

year = "1989",

month = sep,

doi = "10.1007/BF01317927",

language = "English",

volume = "107",

pages = "315--322",

journal = "Archives of Virology",

issn = "0304-8608",

publisher = "Springer Wien",

number = "3-4",

}

TY - JOUR

T1 - Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

AU - Gerna, G.

AU - Sarasini, Antonella

AU - Torsellini, Maria

AU - di Matteo, Angela

AU - Baldanti, F.

AU - Parea, M.

AU - Battaglia, M.

PY - 1989/9

Y1 - 1989/9

N2 - Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

AB - Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

UR - http://www.scopus.com/inward/record.url?scp=0024425112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024425112&partnerID=8YFLogxK

U2 - 10.1007/BF01317927

DO - 10.1007/BF01317927

M3 - Article

C2 - 2554860

AN - SCOPUS:0024425112

VL - 107

SP - 315

EP - 322

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 3-4

ER -

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this