Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

Research output: Contribution to journalArticle

Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalArchives of Virology
Volume107
Issue number3-4
DOIs
Publication statusPublished - Sep 1989

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

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