Characterization of SA-11 rotavirus receptorial structures on human colon carcinoma cell line HT-29

F. Superti, G. Donelli

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The involvement of different cell membrane components in the receptor structures for SA-11 rotavirus was investigated. As experimental model, the human enterocyte-like HT-29 cell line, was used because of its closer resemblance to the in vivo viral cellular target as compared to other in vitro systems. Rotavirus was incubated with whole membranes or their separated protein and lipid fractions before infection. Either isolated cell membranes or lipid components were capable of binding to the virus and to prevent infection, whereas proteins did not show any inhibitory activity. Among lipids, the glycolipid fraction was shown to impede rotaviral antigen synthesis with a dose-dependent relationship, whereas phospholipids failed to prevent viral infection. To confirm these findings, membranes and target cells were subjected to different enzymatic treatments prior to infection. In addition, HT-29 cells were also incubated with different lectins before infection. The blocking activity of membranes was inhibited by treatment with ceramide glycanase, neuraminidase, and beta-galactosidase but not by treatment with proteases or heat (100°C). Viral infection was prevented by preincubation of target cells with lectins specific for sialic acid and galactose or with ceramide glycanase, neuraminidase, and beta-galactosidase, whereas protease treatments were not active. The results of these experimental procedures indicate that glycolipids containing specific carbohydrate moieties, such as sialic acid and galactose, contribute to the SA-11 rotavirus receptor structure on HT-29 cells.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalJournal of Medical Virology
Volume47
Issue number4
DOIs
Publication statusPublished - 1995

Fingerprint

Rotavirus
HT29 Cells
Colon
Carcinoma
Cell Line
Glycolipids
Neuraminidase
N-Acetylneuraminic Acid
Virus Diseases
beta-Galactosidase
Infection
Galactose
Lectins
Peptide Hydrolases
Cell Membrane
Lipids
Virus Attachment
Membranes
Enterocytes
Cellular Structures

Keywords

  • Glycolipids
  • Receptors
  • Virus

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of SA-11 rotavirus receptorial structures on human colon carcinoma cell line HT-29. / Superti, F.; Donelli, G.

In: Journal of Medical Virology, Vol. 47, No. 4, 1995, p. 421-428.

Research output: Contribution to journalArticle

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