A new method which combines in a single sensitive preparation fractionation of serum proteins by thin-layer gel filtration on Sephadex G 200 superfine and the immunological characterization is described. This method applies cellulose acetate membranes onto the surface of a Sephadex thin-layer gel on which the protein fractions have been separated. Strips of filter paper soaked with antiserum are placed parallel and to either side of the membranes removed from the gel. Immnnoprecipitation is allowed to develop for 48 hours in a bath of mineral oil. In normal serum at least 12 different components can be shown.
|Number of pages||8|
|Journal||The Journal of Laboratory and Clinical Medicine|
|Publication status||Published - Mar 1967|
ASJC Scopus subject areas
- Pathology and Forensic Medicine