Characterization of serum proteins by thin-layer gel filtration combined with immunodiffusion

A. Agostoni, C. Vergani, B. Lomanto

Research output: Contribution to journalArticle

Abstract

A new method which combines in a single sensitive preparation fractionation of serum proteins by thin-layer gel filtration on Sephadex G 200 superfine and the immunological characterization is described. This method applies cellulose acetate membranes onto the surface of a Sephadex thin-layer gel on which the protein fractions have been separated. Strips of filter paper soaked with antiserum are placed parallel and to either side of the membranes removed from the gel. Immnnoprecipitation is allowed to develop for 48 hours in a bath of mineral oil. In normal serum at least 12 different components can be shown.

Original languageEnglish
Pages (from-to)522-529
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume69
Issue number3
Publication statusPublished - Mar 1967

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Fingerprint Dive into the research topics of 'Characterization of serum proteins by thin-layer gel filtration combined with immunodiffusion'. Together they form a unique fingerprint.

  • Cite this