Characterization of tau proteins in human neuroblastoma SH-SY5Y cell line

Daniela Uberti, Claudia Rizzini, PierFranco Spano, Maurizio Memo

Research output: Contribution to journalArticlepeer-review


Here we report three experimental paradigms in which tau proteins are differentially localized and expressed in human neuroblastoma cells SH-SY5Y. We found that in undifferentiated cells, tau proteins were predominantly localized in the nucleus. Western blot analysis of nuclear extracts revealed, among the others, a high molecular weight tau isoform and evaluation of tau mRNA levels showed a single tau isoform. After differentiation, tau immunoreactivity was detected only in cytosol and along neuritic processes. The high molecular weight tau isoform disappeared and an additional tau mRNA species was detected. Treatment of differentiated cells with doxorubicin or okadaic acid resulted in an increase of tau immunoreactivity and in a subsequent cell loss. Our results indicate that both subcellular localization and pattern of expression of tau proteins vary depending on the developmental and functional state of the cells, thus suggesting different roles in cell function.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalNeuroscience Letters
Issue number3
Publication statusPublished - Oct 17 1997


  • Doxorubicin
  • Human neuroblastoma SH-SY5Y
  • Okadaic acid
  • Polymerase chain reaction
  • Tau proteins

ASJC Scopus subject areas

  • Neuroscience(all)


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