Abstract
A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 663-670 |
| Number of pages | 8 |
| Journal | Virology |
| Volume | 187 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1992 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator. / Natoli, Gioacchino; Avantaggiati, Maria Laura; Balsano, Clara; De Marzio, Elisabetta; Collepardo, Daniela; Elfassi, Emile; Levrero, Massimo.
In: Virology, Vol. 187, No. 2, 1992, p. 663-670.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator
AU - Natoli, Gioacchino
AU - Avantaggiati, Maria Laura
AU - Balsano, Clara
AU - De Marzio, Elisabetta
AU - Collepardo, Daniela
AU - Elfassi, Emile
AU - Levrero, Massimo
PY - 1992
Y1 - 1992
N2 - A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.
AB - A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.
UR - http://www.scopus.com/inward/record.url?scp=0026588451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026588451&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(92)90469-6
DO - 10.1016/0042-6822(92)90469-6
M3 - Article
C2 - 1546461
AN - SCOPUS:0026588451
VL - 187
SP - 663
EP - 670
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -