TY - JOUR
T1 - Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator
AU - Natoli, Gioacchino
AU - Avantaggiati, Maria Laura
AU - Balsano, Clara
AU - De Marzio, Elisabetta
AU - Collepardo, Daniela
AU - Elfassi, Emile
AU - Levrero, Massimo
PY - 1992
Y1 - 1992
N2 - A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.
AB - A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.
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U2 - 10.1016/0042-6822(92)90469-6
DO - 10.1016/0042-6822(92)90469-6
M3 - Article
C2 - 1546461
AN - SCOPUS:0026588451
VL - 187
SP - 663
EP - 670
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -