Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator

Gioacchino Natoli, Maria Laura Avantaggiati, Clara Balsano, Elisabetta De Marzio, Daniela Collepardo, Emile Elfassi, Massimo Levrero

Research output: Contribution to journalArticlepeer-review

Abstract

A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.

Original languageEnglish
Pages (from-to)663-670
Number of pages8
JournalVirology
Volume187
Issue number2
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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