Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator

Gioacchino Natoli, Maria Laura Avantaggiati, Clara Balsano, Elisabetta De Marzio, Daniela Collepardo, Emile Elfassi, Massimo Levrero

Research output: Contribution to journalArticle

Abstract

A transactivating function generated by carboxy-terminal truncation of the HBV envelope proteins has been recently described. To characterize the preS/S protein domains responsible for transactivation, preS1/S2/S and preS2/S 3′ deletion mutants under the control of the adenoviral major late promoter were tested for their transactivating potential in cotransfection experiments using the c-myc and c-fos regulatory sequences as targets. Deletion of the carboxyterminal hydrophobic domain of the S protein and the presence of the endoplasmic reticulum insertion signal I (ER signal I) are required for the generation of the preS/S transactivating function. Multiple transcription factors binding sites (i.e., TRE, SIRE, and NFkB sites) mediated the truncated preS/S-induced activation of the target regulatory sequences. The transactivation phenomenon is linked, at least in part, to the protein kinase C signaling pathway.

Original languageEnglish
Pages (from-to)663-670
Number of pages8
JournalVirology
Volume187
Issue number2
DOIs
Publication statusPublished - 1992

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Trans-Activators
Hepatitis B virus
Transcriptional Activation
Protein S
Endoplasmic Reticulum
Protein Kinase C
Transcription Factors
Binding Sites
Proteins
Protein Domains

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Natoli, G., Avantaggiati, M. L., Balsano, C., De Marzio, E., Collepardo, D., Elfassi, E., & Levrero, M. (1992). Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator. Virology, 187(2), 663-670. https://doi.org/10.1016/0042-6822(92)90469-6

Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator. / Natoli, Gioacchino; Avantaggiati, Maria Laura; Balsano, Clara; De Marzio, Elisabetta; Collepardo, Daniela; Elfassi, Emile; Levrero, Massimo.

In: Virology, Vol. 187, No. 2, 1992, p. 663-670.

Research output: Contribution to journalArticle

Natoli, G, Avantaggiati, ML, Balsano, C, De Marzio, E, Collepardo, D, Elfassi, E & Levrero, M 1992, 'Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator', Virology, vol. 187, no. 2, pp. 663-670. https://doi.org/10.1016/0042-6822(92)90469-6
Natoli, Gioacchino ; Avantaggiati, Maria Laura ; Balsano, Clara ; De Marzio, Elisabetta ; Collepardo, Daniela ; Elfassi, Emile ; Levrero, Massimo. / Characterization of the hepatitis B virus preS/S region encoded transcriptional transactivator. In: Virology. 1992 ; Vol. 187, No. 2. pp. 663-670.
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