Characterization of the human polymeric immunoglobulin receptor (PIGR) 3′UTR and differential expression of PIGR mRNA during colon tumorigenesis

June L. Traicoff, Laura De Marchis, Britten L. Ginsburg, Rodolfo E. Zamora, Nada H. Khattar, Vincent J. Blanch, Sarah Plummer, Sharon A. Bargo, Dennis J. Templeton, Graham Casey, Charlotte S. Kaetzel

Research output: Contribution to journalArticlepeer-review

Abstract

The human cell lines VACO-235 and VACO-411 constitute a novel in vitro model of colon adenoma to carcinoma progression. By differential display RT-PCR we identified a transcript that is expressed in the parental nontumorigenic adenoma line (VACO-235E), but is not expressed in the tumorigenic daughter (VACO-235L) or granddaughter (VACO-411) lines. This cDNA represents a previously uncharacterized portion of the 3′UTR of human PIGR. Human PIGR mRNA was found to be highly expressed in normal colon epithelium, but was decreased in 6 of 8 colon tumors and was negligible in 8 of 10 colon tumor cell lines. We sequenced the entire 1.8 kb 3′UTR of human PIGR, and found it to contain multiple repetitive elements as well as elements that could affect the processing and stability of PIGR mRNA. We hypothesize that differential regulation of PIGR mRNA stability may contribute to its downregulation in colon cancer.

Original languageEnglish
Pages (from-to)792-804
Number of pages13
JournalJournal of Biomedical Science
Volume10
Issue number6 II
DOIs
Publication statusPublished - 2003

Keywords

  • 3′UTR
  • Adenoma
  • Colon cancer
  • MRNA differential display
  • Neoplastic cell transformation
  • Polymeric immunoglobulin receptor
  • RNA stability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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