Characterization of the human STAT5A and STAT5B promoters: Evidence of a positive and negative mechanism of transcriptional regulation

Stefania Crispi, Emma Sanzari, Jlenia Monfregola, Nicola De Felice, Giorgia Fimiani, Raffaele Ambrosio, Michele D'Urso, Matilde Valeria Ursini

Research output: Contribution to journalArticlepeer-review

Abstract

We recently published the genomic characterization of the STAT5A and STAT5B paralogous genes that are located head to head in the 17q21 chromosome and share large regions of sequence identity. We here demonstrate by transient in vitro transfection that STAT5A and STAT5B promoters are able to direct comparable levels of transcription. The expression of basal promoters is enhanced after Sp1 up-regulation in HeLa and SL2 cells while DNA methylation associated to the recruitment of MeCP2 methyl CpG binding protein down-regulates STAT5A and B promoters by interfering with Sp1-induced transcription. In addition, cross-species sequence comparison identified a bi-directional negative cis-acting regulatory element located in the STAT5 intergenic region.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalFEBS Letters
Volume562
Issue number1-3
DOIs
Publication statusPublished - Mar 26 2004

Keywords

  • Cis-acting element
  • Cross-species comparison
  • Epigenetic control
  • GM-CSF, granulocyte-macrophage colony-stimulating factor
  • IL-2, interleukin 2
  • STAT, signal transducer and activator of transcription
  • STAT5A
  • STAT5B
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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