Characterization of the immune response of human cord-blood derived γδ T cells to stimulation with aminobisphosphonate compounds

R. Placido, G. Auricchio, I. Gabriele, E. Galli, E. Brunetti, V. Colizzi, L. Battistini, Giorgio Mancino

Research output: Contribution to journalArticle

Abstract

Vγ9Vδ2 T lymphocytes have been shown to respond to a variety of non-peptide antigens including alkylamines and phosphoantigens. Recently, aminobisphosphonates have also been shown to stimulate this subset of γδ+ T cells. In this study we analyzed the proliferative responses of freshly isolated γδ T lymphocytes obtained from human cord blood when challenged with pyrophosphomonoesters or aminobisphosphonates. Nitrogen-containing aminobisphopsphonates, in contrast to phoshoantigens, readily stimulated expansion of Vδ2Vγ9 cells in human cord blood. Expanded cells displayed an activated mature phenotype, and were capable of producing TNFα and IFNγ but not perforin following secondary stimulation, consistent with the development of a regulatory, as opposed to cytotoxic, phenotype. This approach may provide a useful strategy for a new approach to the treatment of neonatal pathologies.

Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number1
Publication statusPublished - Jan 2011

Keywords

  • γδ T cells
  • Aminobisphosphonates
  • Cord blood
  • Regulatory phenotype

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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