Characterization of the major allergen of plum as a lipid transfer protein

Elide A. Pastorello, Laura Farioli, Valerio Pravettoni, Maria Gabriella Giuffrida, Claudio Ortolani, Donatella Fortunato, Chiara Trambaioli, Elisabetta Scibola, Ambra M. Calamari, Anna M. Robino, Amedeo Conti

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Allergy to Prunoideae fruit (plum, peach, cherry and apricot) is one of the most frequent food allergies in southern Europe. All these fruits cross-react in vivo and in vitro, as they share their major allergen, a 9 kD lipid transfer protein (LTP). Objective: The aim of the study was the identification and molecular characterization of the major allergen of plum. Methods: The IgE pattern of reactivity to plums was investigated by SDS-PAGE and immunoblotting with the sera of 23 patients. The identified major allergen was purified by HPLC, using a cationic-exchange column followed by gel-filtration. Further characterization was achieved by periodic-Schiff stain, isoelectrofocusing and N-terminal amino acid sequencing. Results and conclusions: The major allergen of plum is a 9 kD lipid transfer protein, not glycosylated and with a basic character (pI>9), highly homologous to the major allergen of peach.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume756
Issue number1-2
DOIs
Publication statusPublished - May 25 2001

Keywords

  • Food allergy
  • Lipid transfer proteins
  • Prunoideae fruit
  • Skin prick test

ASJC Scopus subject areas

  • Chemistry(all)

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