Characterization of the neuroligin gene family expression and evolution in zebrafish.

Alberto Rissone, Lorenzo Sangiorgio, Matteo Monopoli, Monica Beltrame, Ileana Zucchi, Federico Bussolino, Marco Arese, Franco Cotelli

Research output: Contribution to journalArticle

Abstract

Neuroligins constitute a family of transmembrane proteins localized at the postsynaptic side of both excitatory and inhibitory synapses of the central nervous system. They are involved in synaptic function and maturation and recent studies have linked mutations in specific human Neuroligins to mental retardation and autism. We isolated the human Neuroligin homologs in Danio rerio. Next, we studied their gene structures and we reconstructed the evolution of the Neuroligin genes across vertebrate phyla. Using reverse-transcriptase polymerase chain reaction, we analyzed the expression and alternative splicing pattern of each gene during zebrafish embryonic development and in different adult organs. By in situ hybridization, we analyzed the temporal and spatial expression pattern during embryonic development and larval stages and we found that zebrafish Neuroligins are expressed throughout the nervous system. Globally, our results indicate that, during evolution, specific subfunctionalization events occurred within paralogous members of this gene family in zebrafish.

Original languageEnglish
Pages (from-to)688-702
Number of pages15
JournalDevelopmental Dynamics
Volume239
Issue number2
DOIs
Publication statusPublished - Feb 2010

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Zebrafish
Gene Expression
Genes
Embryonic Development
Alternative Splicing
Autistic Disorder
Reverse Transcriptase Polymerase Chain Reaction
Intellectual Disability
Synapses
Nervous System
In Situ Hybridization
Vertebrates
Central Nervous System
Mutation
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rissone, A., Sangiorgio, L., Monopoli, M., Beltrame, M., Zucchi, I., Bussolino, F., ... Cotelli, F. (2010). Characterization of the neuroligin gene family expression and evolution in zebrafish. Developmental Dynamics, 239(2), 688-702. https://doi.org/10.1002/dvdy.22196

Characterization of the neuroligin gene family expression and evolution in zebrafish. / Rissone, Alberto; Sangiorgio, Lorenzo; Monopoli, Matteo; Beltrame, Monica; Zucchi, Ileana; Bussolino, Federico; Arese, Marco; Cotelli, Franco.

In: Developmental Dynamics, Vol. 239, No. 2, 02.2010, p. 688-702.

Research output: Contribution to journalArticle

Rissone, A, Sangiorgio, L, Monopoli, M, Beltrame, M, Zucchi, I, Bussolino, F, Arese, M & Cotelli, F 2010, 'Characterization of the neuroligin gene family expression and evolution in zebrafish.', Developmental Dynamics, vol. 239, no. 2, pp. 688-702. https://doi.org/10.1002/dvdy.22196
Rissone, Alberto ; Sangiorgio, Lorenzo ; Monopoli, Matteo ; Beltrame, Monica ; Zucchi, Ileana ; Bussolino, Federico ; Arese, Marco ; Cotelli, Franco. / Characterization of the neuroligin gene family expression and evolution in zebrafish. In: Developmental Dynamics. 2010 ; Vol. 239, No. 2. pp. 688-702.
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