Characterization of the P373L E-cadherin germline missense mutation and implication for clinical management

G. Corso, F. Roviello, J. Paredes, C. Pedrazzani, M. Novais, J. Correia, D. Marrelli, L. Cirnes, R. Seruca, C. Oliveira, G. Suriano

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Hereditary diffuse gastric cancer (HDGC) is a cancer susceptibility syndrome caused by E-cadherin germline mutations. One-third of these mutations are of the missense type, representing a burden in genetic counselling. A new germline missense mutation (P373L) was recently identified in a HDGC Italian family. The present work aimed at addressing the disease-causative nature of the P373L mutant. Methods: Assessment of the P373L mutation effect was based on cell aggregation and invasion assays. LOH analysis at the E-cadherin locus, search for somatic E-cadherin mutations and for promoter hypermethylation were performed to identify the mechanism of inactivation of the E-cadherin wild-type allele in the tumour. Results: In vitro the P373L germline mutation impaired the E-cadherin functions. E-cadherin promoter hypermethylation was observed in the tumour of the P373L mutation carrier. Conclusion: We conclude that the combination of clinical, in vitro and molecular genetic data is helpful for establishing an accurate analysis of HDGC-associated CDH1 germline missense mutations and subsequently for appropriate clinical management of asymptomatic mutation carriers.

Original languageEnglish
Pages (from-to)1061-1067
Number of pages7
JournalEuropean Journal of Surgical Oncology
Volume33
Issue number9
DOIs
Publication statusPublished - Nov 2007

Keywords

  • Clinical management
  • E-cadherin germline missense mutation
  • HDGC
  • In vitro analysis
  • Second inactivating hit

ASJC Scopus subject areas

  • Oncology
  • Surgery

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