Characterization of the Pall Celeris system as a point-of-care device for therapeutic angiogenesis

Gabriella Spaltro, Stefania Straino, Elisa Gambini, Beatrice Bassetti, Luca Persico, Stefano Zoli, Marco Zanobini, Maurizio C. Capogrossi, Rita Spirito, Cinzia Quarti, Giulio Pompilio

Research output: Contribution to journalArticlepeer-review

Abstract

Background aims: The Pall Celeris system is a filtration-based point-of-care device designed to obtain a high concentrate of peripheral blood total nucleated cells (PB-TNCs). We have characterized the Pall Celeris-derived TNCs for their in vitro and in vivo angiogenic potency. Methods: PB-TNCs isolated from healthy donors were characterized through the use of flow cytometry and functional assays, aiming to assess migratory capacity, ability to form capillary-like structures, endothelial trans-differentiation and paracrine factor secretion. In a hind limb ischemia mouse model, we evaluated perfusion immediately and 7 days after surgery, along with capillary, arteriole and regenerative fiber density and local bio-distribution. Results: Human PB-TNCs isolated by use of the Pall Celeris filtration system were shown to secrete a panel of angiogenic factors and migrate in response to vascular endothelial growth factor and stromal-derived factor-1 stimuli. Moreover, after injection in a mouse model of hind limb ischemia, PB-TNCs induced neovascularization by increasing capillary, arteriole and regenerative fiber numbers, with human cells detected in murine tissue up to 7 days after ischemia. Conclusions: The Pall Celeris system may represent a novel, effective and reliable point-of-care device to obtain a PB-derived cell product with adequate potency for therapeutic angiogenesis.

Original languageEnglish
Pages (from-to)1302-1313
Number of pages12
JournalCytotherapy
Volume17
Issue number9
DOIs
Publication statusPublished - 2015

Keywords

  • Angiogenesis
  • Hind limb ischemia
  • Peripheral blood cells
  • Point-of-care

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Oncology
  • Genetics(clinical)
  • Transplantation
  • Cancer Research
  • Cell Biology

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