Characterization of the T-cell epitopes of the major peach allergen Pru p 3

E. A. Pastorello, M. Monza, V. Pravettoni, R. Longhi, P. Bonara, J. Scibilia, L. Primavesi, R. Scorza

Research output: Contribution to journalArticle

Abstract

Background: Pru p 3 is the major peach allergen recognized by more than 90% of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≥5). These TCLs proliferated mainly in response to Pru p 312-27 and Pru p 357-72. Pru-p-3-specific TCLs were mainly CD4+ (81%) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 312-27 and Pru p 3 57-72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalInternational Archives of Allergy and Immunology
Volume153
Issue number1
DOIs
Publication statusPublished - Aug 2010

Fingerprint

T-Lymphocyte Epitopes
T-Lymphocytes
Cell Line
Phenotype
Interleukin-4
Pru p 3 allergen
Prunus persica
Peptides
Immunotherapy
Allergens
Interferons
Blood Cells
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Cytokines
Antigens

Keywords

  • γ-Interferon
  • Food allergy
  • Interleukin 4
  • Lipid transfer protein
  • Peach allergy
  • Pru p 3 allergen
  • T-cell epitopes
  • Th profile

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Characterization of the T-cell epitopes of the major peach allergen Pru p 3. / Pastorello, E. A.; Monza, M.; Pravettoni, V.; Longhi, R.; Bonara, P.; Scibilia, J.; Primavesi, L.; Scorza, R.

In: International Archives of Allergy and Immunology, Vol. 153, No. 1, 08.2010, p. 1-12.

Research output: Contribution to journalArticle

Pastorello, EA, Monza, M, Pravettoni, V, Longhi, R, Bonara, P, Scibilia, J, Primavesi, L & Scorza, R 2010, 'Characterization of the T-cell epitopes of the major peach allergen Pru p 3', International Archives of Allergy and Immunology, vol. 153, no. 1, pp. 1-12. https://doi.org/10.1159/000301573
Pastorello, E. A. ; Monza, M. ; Pravettoni, V. ; Longhi, R. ; Bonara, P. ; Scibilia, J. ; Primavesi, L. ; Scorza, R. / Characterization of the T-cell epitopes of the major peach allergen Pru p 3. In: International Archives of Allergy and Immunology. 2010 ; Vol. 153, No. 1. pp. 1-12.
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abstract = "Background: Pru p 3 is the major peach allergen recognized by more than 90{\%} of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≥5). These TCLs proliferated mainly in response to Pru p 312-27 and Pru p 357-72. Pru-p-3-specific TCLs were mainly CD4+ (81{\%}) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 312-27 and Pru p 3 57-72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.",
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T1 - Characterization of the T-cell epitopes of the major peach allergen Pru p 3

AU - Pastorello, E. A.

AU - Monza, M.

AU - Pravettoni, V.

AU - Longhi, R.

AU - Bonara, P.

AU - Scibilia, J.

AU - Primavesi, L.

AU - Scorza, R.

PY - 2010/8

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N2 - Background: Pru p 3 is the major peach allergen recognized by more than 90% of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≥5). These TCLs proliferated mainly in response to Pru p 312-27 and Pru p 357-72. Pru-p-3-specific TCLs were mainly CD4+ (81%) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 312-27 and Pru p 3 57-72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.

AB - Background: Pru p 3 is the major peach allergen recognized by more than 90% of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≥5). These TCLs proliferated mainly in response to Pru p 312-27 and Pru p 357-72. Pru-p-3-specific TCLs were mainly CD4+ (81%) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 312-27 and Pru p 3 57-72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.

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KW - T-cell epitopes

KW - Th profile

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