Characterization of transformed cell lines evolving from a normal c3h line

Laura Curatolo, Gianni Frascotti, Paolo Ubezio, Cecilia Mannironi, Luciano Morasca

Research output: Contribution to journalArticlepeer-review


The development of transformed cell lines evolving from an embryo fibroblastic C3H primary culture was followed before and after the ageing crisis using different techniques. By flow cytometry, alteration of subpopulations having different DNA content and altered metabolic activity was observed after the crisis, with the trend to assume a near tetraploid DNA index at higher passages. The fibrin clot retractile activity was lost in all cases during the ageing crisis, but the outcome did not present uniform values of growth characteristics or chromosome number and tumorigenicity appeared to be a nonstable property of the transformed cell lines.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
Issue number1-2
Publication statusPublished - 1988


  • Fibrin clot retraction
  • Fibroblasts
  • Flow cytometry
  • Transformation
  • Tumorigenicity

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pathology and Forensic Medicine


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