Abstract
NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.
| Original language | English |
|---|---|
| Pages (from-to) | 1433-1438 |
| Number of pages | 6 |
| Journal | Journal of Virology |
| Volume | 70 |
| Issue number | 3 |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Immunology
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Characterization of two novel YY1 binding sites in the polyomavirus late promoter. / Martelli, Fabio; Iacobini, Carla; Caruso, Maurizia; Felsani, Armando.
In: Journal of Virology, Vol. 70, No. 3, 1996, p. 1433-1438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of two novel YY1 binding sites in the polyomavirus late promoter
AU - Martelli, Fabio
AU - Iacobini, Carla
AU - Caruso, Maurizia
AU - Felsani, Armando
PY - 1996
Y1 - 1996
N2 - NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.
AB - NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030071006&partnerID=8YFLogxK
M3 - Article
C2 - 8627660
AN - SCOPUS:0030071006
VL - 70
SP - 1433
EP - 1438
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 3
ER -