Characterization of two novel YY1 binding sites in the polyomavirus late promoter

Fabio Martelli, Carla Iacobini, Maurizia Caruso, Armando Felsani

Research output: Contribution to journalArticle

Abstract

NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.

Original languageEnglish
Pages (from-to)1433-1438
Number of pages6
JournalJournal of Virology
Volume70
Issue number3
Publication statusPublished - 1996

Fingerprint

Polyomavirus
binding sites
Binding Sites
promoter regions
Nucleotide Motifs
Nucleic Acid Regulatory Sequences
Transcription Initiation Site
site-directed mutagenesis
DNA
Site-Directed Mutagenesis
cooperatives
Transcription Factors
transcription factors
transcription (genetics)
cell lines
Genome
Cell Line
genome

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of two novel YY1 binding sites in the polyomavirus late promoter. / Martelli, Fabio; Iacobini, Carla; Caruso, Maurizia; Felsani, Armando.

In: Journal of Virology, Vol. 70, No. 3, 1996, p. 1433-1438.

Research output: Contribution to journalArticle

Martelli, F, Iacobini, C, Caruso, M & Felsani, A 1996, 'Characterization of two novel YY1 binding sites in the polyomavirus late promoter', Journal of Virology, vol. 70, no. 3, pp. 1433-1438.
Martelli, Fabio ; Iacobini, Carla ; Caruso, Maurizia ; Felsani, Armando. / Characterization of two novel YY1 binding sites in the polyomavirus late promoter. In: Journal of Virology. 1996 ; Vol. 70, No. 3. pp. 1433-1438.
@article{39e9f46fa9cf40d1a5dd07e9336c2f64,
title = "Characterization of two novel YY1 binding sites in the polyomavirus late promoter",
abstract = "NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.",
author = "Fabio Martelli and Carla Iacobini and Maurizia Caruso and Armando Felsani",
year = "1996",
language = "English",
volume = "70",
pages = "1433--1438",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Characterization of two novel YY1 binding sites in the polyomavirus late promoter

AU - Martelli, Fabio

AU - Iacobini, Carla

AU - Caruso, Maurizia

AU - Felsani, Armando

PY - 1996

Y1 - 1996

N2 - NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.

AB - NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, δ, NF-E1, UCRBP, or CF1. Moreover, we show the presence in the polyomavirus genome of a second DNA motif, located 40 bp from the first, which binds YY1/NF-D with high affinity. Both sites lie downstream of the major late transcription initiation sites. By site-directed mutagenesis, we demonstrate that both elements contribute positively to the activity of the late promoter, probably by a cooperative mechanism. We also demonstrate that the requirement of the YY1/NF-D function for late promoter activity varies with the cell line.

UR - http://www.scopus.com/inward/record.url?scp=0030071006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030071006&partnerID=8YFLogxK

M3 - Article

C2 - 8627660

AN - SCOPUS:0030071006

VL - 70

SP - 1433

EP - 1438

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 3

ER -