Characterization of unusual G8 rotavirus strains isolated from Egyptian children

J. L. Holmes, C. D. Kirkwood, G. Gerna, J. D. Clemens, M. R. Rao, A. B. Naficy, R. Abu-Elyazeed, S. J. Savarino, R. I. Glass, J. R. Gentsch

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We report the first detection of P[14], G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of the genes encoding VP7 and the VP8 portion of the VP4 gene. These two strains shared a high level of homology of their VP7s (87.8% nucleotide [nt], 97.2% amino acid [aa]) and VP4s (89.6% nt, 97.1% aa) and had the highest VP7 identity to serotype G8 (>82% nt, >92% aa) and VP4 identity to genotype P[14] (>81% nt, >91% aa) strains. Serological results with a VP7 G8-specific and VP4 P[14]-specific neutralizing monoclonal antibodies supported the genetic classification of EGY1850 and EGY2295 as P[14], G8. Genogroup analysis supports earlier findings that human G8 rotaviruses may be genetically related to bovine rotaviruses. These findings demonstrate that our understanding of the geographic distribution of rotavirus strains is incomplete, emphasize the need to monitor rotavirus serotypes, and extend the known distribution of serotype G8 and genotype P[14] strains in Africa.

Original languageEnglish
Pages (from-to)1381-1396
Number of pages16
JournalArchives of Virology
Volume144
Issue number7
DOIs
Publication statusPublished - 1999

Fingerprint

Rotavirus
Nucleotides
Amino Acids
Genotype
Serotyping
Egypt
Neutralizing Antibodies
Immunoenzyme Techniques
Genes
Sequence Analysis
Epidemiology
Monoclonal Antibodies
Viruses
Serogroup

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Holmes, J. L., Kirkwood, C. D., Gerna, G., Clemens, J. D., Rao, M. R., Naficy, A. B., ... Gentsch, J. R. (1999). Characterization of unusual G8 rotavirus strains isolated from Egyptian children. Archives of Virology, 144(7), 1381-1396. https://doi.org/10.1007/s007050050594

Characterization of unusual G8 rotavirus strains isolated from Egyptian children. / Holmes, J. L.; Kirkwood, C. D.; Gerna, G.; Clemens, J. D.; Rao, M. R.; Naficy, A. B.; Abu-Elyazeed, R.; Savarino, S. J.; Glass, R. I.; Gentsch, J. R.

In: Archives of Virology, Vol. 144, No. 7, 1999, p. 1381-1396.

Research output: Contribution to journalArticle

Holmes, JL, Kirkwood, CD, Gerna, G, Clemens, JD, Rao, MR, Naficy, AB, Abu-Elyazeed, R, Savarino, SJ, Glass, RI & Gentsch, JR 1999, 'Characterization of unusual G8 rotavirus strains isolated from Egyptian children', Archives of Virology, vol. 144, no. 7, pp. 1381-1396. https://doi.org/10.1007/s007050050594
Holmes, J. L. ; Kirkwood, C. D. ; Gerna, G. ; Clemens, J. D. ; Rao, M. R. ; Naficy, A. B. ; Abu-Elyazeed, R. ; Savarino, S. J. ; Glass, R. I. ; Gentsch, J. R. / Characterization of unusual G8 rotavirus strains isolated from Egyptian children. In: Archives of Virology. 1999 ; Vol. 144, No. 7. pp. 1381-1396.
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