Abstract
Thus far, the methods used to determine erythrocyte Ca2+ influx have not allowed the assessment of the kinetics of ion uptake. To overcome this drawback, we studied a new method, using the fluorescent Ca2+-chelator fura-2, which directly quantifies intracellular Ca2+ changes in human erythrocytes. This method has the advantage over previous techniques that it monitors continuously cellular Ca2+ levels. The Ca2+ influx is modulated by cellular membrane potential in the presence of a transmembrane Ca2+ concentration gradient and exhibits a first slow increase of the intracellular Ca2+ concentration, followed, after the reachment of a threshold value of 125 ± 13 nM Ca2+, by a faster increase until a plateau is reached. The influx rate is inhibited by dihydropyridines in the micromolar range. These findings support the hypothesis that erythrocyte Ca2+ influx is mediated by a carrier similar to the slow Ca2+ channels and is dependent on membrane depolarization.
| Original language | English |
|---|---|
| Pages (from-to) | 549-554 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 236 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 30 1997 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Characterization of voltage-dependent calcium influx in human erythrocytes by fura-2. / Soldati, Laura; Spaventa, Renato; Vezzoli, Giuseppe; Zerbi, Simona; Adamo, Donatella; Caumo, Andrea; Rivera, Rodolfo; Bianchi, Giuseppe.
In: Biochemical and Biophysical Research Communications, Vol. 236, No. 3, 30.07.1997, p. 549-554.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of voltage-dependent calcium influx in human erythrocytes by fura-2
AU - Soldati, Laura
AU - Spaventa, Renato
AU - Vezzoli, Giuseppe
AU - Zerbi, Simona
AU - Adamo, Donatella
AU - Caumo, Andrea
AU - Rivera, Rodolfo
AU - Bianchi, Giuseppe
PY - 1997/7/30
Y1 - 1997/7/30
N2 - Thus far, the methods used to determine erythrocyte Ca2+ influx have not allowed the assessment of the kinetics of ion uptake. To overcome this drawback, we studied a new method, using the fluorescent Ca2+-chelator fura-2, which directly quantifies intracellular Ca2+ changes in human erythrocytes. This method has the advantage over previous techniques that it monitors continuously cellular Ca2+ levels. The Ca2+ influx is modulated by cellular membrane potential in the presence of a transmembrane Ca2+ concentration gradient and exhibits a first slow increase of the intracellular Ca2+ concentration, followed, after the reachment of a threshold value of 125 ± 13 nM Ca2+, by a faster increase until a plateau is reached. The influx rate is inhibited by dihydropyridines in the micromolar range. These findings support the hypothesis that erythrocyte Ca2+ influx is mediated by a carrier similar to the slow Ca2+ channels and is dependent on membrane depolarization.
AB - Thus far, the methods used to determine erythrocyte Ca2+ influx have not allowed the assessment of the kinetics of ion uptake. To overcome this drawback, we studied a new method, using the fluorescent Ca2+-chelator fura-2, which directly quantifies intracellular Ca2+ changes in human erythrocytes. This method has the advantage over previous techniques that it monitors continuously cellular Ca2+ levels. The Ca2+ influx is modulated by cellular membrane potential in the presence of a transmembrane Ca2+ concentration gradient and exhibits a first slow increase of the intracellular Ca2+ concentration, followed, after the reachment of a threshold value of 125 ± 13 nM Ca2+, by a faster increase until a plateau is reached. The influx rate is inhibited by dihydropyridines in the micromolar range. These findings support the hypothesis that erythrocyte Ca2+ influx is mediated by a carrier similar to the slow Ca2+ channels and is dependent on membrane depolarization.
UR - http://www.scopus.com/inward/record.url?scp=0031591691&partnerID=8YFLogxK
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U2 - 10.1006/bbrc.1997.7002
DO - 10.1006/bbrc.1997.7002
M3 - Article
C2 - 9245686
AN - SCOPUS:0031591691
VL - 236
SP - 549
EP - 554
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -