Che-1

A new effector of checkpoints signaling

Aristide Floridi, Maurizio Fanciulli

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Che-1 is a RNA polymerase II binding protein involved in the transcriptional regulation of E2F target-genes and in cell proliferation. Recently, it has been shown that Che-1 accumulates in cells responding to genotoxic agents, such as Doxorubicin and ionizing radiations. The DNA damage-activated checkpoint kinases ATM and Chk2 interact with and phosphorylate Che-1, enhancing its accumulation and stability, and promoting Che-1-mediated transcription of p53-responsive genes and of p53 itself, as evidenced by microarray analysis. This transcriptional response is suppressed by expression of a Che-1 mutant lacking ATM and Chk2 phosphorylation amino acid residues, or by depletion of Che-1 by RNA silencing. In addition, chromatin immunoprecipitation analysis has shown that Che-1 is released from the E2F-target genes and recruited to the p21 and p53 promoters after DNA damage. Lastly, Che-1 contributes to the maintenance of the G2/M checkpoint in response to genotoxic stresses. These findings identify a new mechanism by which the checkpoint kinases regulate, via the novel effector Che-1, the p53 pathway.

Original languageEnglish
Pages (from-to)804-806
Number of pages3
JournalCell Cycle
Volume6
Issue number7
Publication statusPublished - Apr 1 2007

Fingerprint

DNA Damage
Genes
Automatic teller machines
Phosphotransferases
Phosphorylation
Chromatin Immunoprecipitation
DNA
Ionizing radiation
p53 Genes
Cell proliferation
Transcription
Microarray Analysis
Microarrays
RNA Interference
Ionizing Radiation
Doxorubicin
Chromatin
Maintenance
Cell Proliferation
RNA

Keywords

  • AATF
  • ATM
  • Che-1
  • Chk2
  • DNA damage
  • G/M checkpoint
  • p53

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Che-1 : A new effector of checkpoints signaling. / Floridi, Aristide; Fanciulli, Maurizio.

In: Cell Cycle, Vol. 6, No. 7, 01.04.2007, p. 804-806.

Research output: Contribution to journalArticle

Floridi, A & Fanciulli, M 2007, 'Che-1: A new effector of checkpoints signaling', Cell Cycle, vol. 6, no. 7, pp. 804-806.
Floridi, Aristide ; Fanciulli, Maurizio. / Che-1 : A new effector of checkpoints signaling. In: Cell Cycle. 2007 ; Vol. 6, No. 7. pp. 804-806.
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