Abstract
Che-1 is a nuclear protein involved in the regulation of gene transcription and cell proliferation. It has also been shown to localize to the cytoplasm of postmitotic neuronal cells, where it is able to interact with the microtubule-associated protein tau. Cyclin-dependent kinase 5 (Cdk5) is a postmitotic proline-directed serine/threonine kinase that hyperphosphorylates tau under pathological conditions. We observed that Che-1 overexpression induces Cdk5 expression both at the mRNA and protein levels. Furthermore, we show that Che-1 directly interacts with Cdk5 protein in vivo. Cdk5/Che-1 complex formation does not compete with Cdk5/p35 interaction, thus Che-1 is able to bind the active kinase complex. Finally, we demonstrated that Che-1 is itself a Cdk5 substrate.
| Original language | English |
|---|---|
| Pages (from-to) | 531-535 |
| Number of pages | 5 |
| Journal | NeuroReport |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 2008 |
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Keywords
- Alzheimer
- Apoptosis-antagonizing transcription factor
- Che-1
- Cyclin-dependent kinase 5
- p35
- Tau
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex. / Buontempo, Serena; Barbato, Christian; Bruno, Tiziana; Corbi, Nicoletta; Ciotti, Maria Teresa; Floridi, Aristide; Fanciulli, Maurizio; Passananti, Claudio.
In: NeuroReport, Vol. 19, No. 5, 03.2008, p. 531-535.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex
AU - Buontempo, Serena
AU - Barbato, Christian
AU - Bruno, Tiziana
AU - Corbi, Nicoletta
AU - Ciotti, Maria Teresa
AU - Floridi, Aristide
AU - Fanciulli, Maurizio
AU - Passananti, Claudio
PY - 2008/3
Y1 - 2008/3
N2 - Che-1 is a nuclear protein involved in the regulation of gene transcription and cell proliferation. It has also been shown to localize to the cytoplasm of postmitotic neuronal cells, where it is able to interact with the microtubule-associated protein tau. Cyclin-dependent kinase 5 (Cdk5) is a postmitotic proline-directed serine/threonine kinase that hyperphosphorylates tau under pathological conditions. We observed that Che-1 overexpression induces Cdk5 expression both at the mRNA and protein levels. Furthermore, we show that Che-1 directly interacts with Cdk5 protein in vivo. Cdk5/Che-1 complex formation does not compete with Cdk5/p35 interaction, thus Che-1 is able to bind the active kinase complex. Finally, we demonstrated that Che-1 is itself a Cdk5 substrate.
AB - Che-1 is a nuclear protein involved in the regulation of gene transcription and cell proliferation. It has also been shown to localize to the cytoplasm of postmitotic neuronal cells, where it is able to interact with the microtubule-associated protein tau. Cyclin-dependent kinase 5 (Cdk5) is a postmitotic proline-directed serine/threonine kinase that hyperphosphorylates tau under pathological conditions. We observed that Che-1 overexpression induces Cdk5 expression both at the mRNA and protein levels. Furthermore, we show that Che-1 directly interacts with Cdk5 protein in vivo. Cdk5/Che-1 complex formation does not compete with Cdk5/p35 interaction, thus Che-1 is able to bind the active kinase complex. Finally, we demonstrated that Che-1 is itself a Cdk5 substrate.
KW - Alzheimer
KW - Apoptosis-antagonizing transcription factor
KW - Che-1
KW - Cyclin-dependent kinase 5
KW - p35
KW - Tau
UR - http://www.scopus.com/inward/record.url?scp=41849086946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41849086946&partnerID=8YFLogxK
U2 - 10.1097/WNR.0b013e3282f85c1b
DO - 10.1097/WNR.0b013e3282f85c1b
M3 - Article
C2 - 18388733
AN - SCOPUS:41849086946
VL - 19
SP - 531
EP - 535
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 5
ER -