Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex

Serena Buontempo, Christian Barbato, Tiziana Bruno, Nicoletta Corbi, Maria Teresa Ciotti, Aristide Floridi, Maurizio Fanciulli, Claudio Passananti

Research output: Contribution to journalArticle

Abstract

Che-1 is a nuclear protein involved in the regulation of gene transcription and cell proliferation. It has also been shown to localize to the cytoplasm of postmitotic neuronal cells, where it is able to interact with the microtubule-associated protein tau. Cyclin-dependent kinase 5 (Cdk5) is a postmitotic proline-directed serine/threonine kinase that hyperphosphorylates tau under pathological conditions. We observed that Che-1 overexpression induces Cdk5 expression both at the mRNA and protein levels. Furthermore, we show that Che-1 directly interacts with Cdk5 protein in vivo. Cdk5/Che-1 complex formation does not compete with Cdk5/p35 interaction, thus Che-1 is able to bind the active kinase complex. Finally, we demonstrated that Che-1 is itself a Cdk5 substrate.

Original languageEnglish
Pages (from-to)531-535
Number of pages5
JournalNeuroReport
Volume19
Issue number5
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Cyclin-Dependent Kinase 5
Phosphotransferases
Microtubule-Associated Proteins
Protein-Serine-Threonine Kinases
Nuclear Proteins
Proline
Cytoplasm
Proteins
Cell Proliferation
Messenger RNA
Genes

Keywords

  • Alzheimer
  • Apoptosis-antagonizing transcription factor
  • Che-1
  • Cyclin-dependent kinase 5
  • p35
  • Tau

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Buontempo, S., Barbato, C., Bruno, T., Corbi, N., Ciotti, M. T., Floridi, A., ... Passananti, C. (2008). Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex. NeuroReport, 19(5), 531-535. https://doi.org/10.1097/WNR.0b013e3282f85c1b

Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex. / Buontempo, Serena; Barbato, Christian; Bruno, Tiziana; Corbi, Nicoletta; Ciotti, Maria Teresa; Floridi, Aristide; Fanciulli, Maurizio; Passananti, Claudio.

In: NeuroReport, Vol. 19, No. 5, 03.2008, p. 531-535.

Research output: Contribution to journalArticle

Buontempo, S, Barbato, C, Bruno, T, Corbi, N, Ciotti, MT, Floridi, A, Fanciulli, M & Passananti, C 2008, 'Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex', NeuroReport, vol. 19, no. 5, pp. 531-535. https://doi.org/10.1097/WNR.0b013e3282f85c1b
Buontempo, Serena ; Barbato, Christian ; Bruno, Tiziana ; Corbi, Nicoletta ; Ciotti, Maria Teresa ; Floridi, Aristide ; Fanciulli, Maurizio ; Passananti, Claudio. / Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex. In: NeuroReport. 2008 ; Vol. 19, No. 5. pp. 531-535.
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