Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation

Tiziana Bruno, Agata Desantis, Gianluca Bossi, Silvia Di Agostino, Cristina Sorino, Francesca De Nicola, Simona Iezzi, Annapaola Franchitto, Barbara Benassi, Sergio Galanti, Francesca La Rosa, Aristide Floridi, Alfonso Bellacosa, Claudio Passananti, Giovanni Blandino, Maurizio Fanciulli

Research output: Contribution to journalArticlepeer-review

Abstract

Che-1 is a RNA polymerase II binding protein involved in the regulation of gene transcription and, in response to DNA damage, promotes p53 transcription. In this study, we investigated whether Che-1 regulates mutant p53 expression. We found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo. Notably, loss of Che-1 activates DNA damage checkpoint response and induces transactivation of p73. Therefore, these findings underline the important role that Che-1 has in survival of cells expressing mutant p53.

Original languageEnglish
Pages (from-to)122-134
Number of pages13
JournalCancer Cell
Volume18
Issue number2
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Cellcycle

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

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