Chemical characterization, antioxidant and cytotoxic activities of the methanolic extract of Hymenocrater longiflorus grown in Iraq

Rafal S A Al-Anee, Ghassan M. Sulaiman, Khulood W. Al-Sammarrae, Giuliana Napolitano, Renzo Bagnati, Luigi Lania, Alice Passoni, Barbara Majello

Research output: Contribution to journalArticle

Abstract

Hymenocrater longiflorus was collected from northern Iraq, and the chemical composition and antioxidant and cytotoxic activities of this plant were investigated. Ten compounds detected by HPLC-ESI/MS were identified as flavonoids and phenolic acids. The free radical scavenging activity of the 70% methanol extract was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The antioxidant activities of the extract may be attributed to its polyphenolic composition. The cytotoxicity of the plant extract against the osteosarcoma (U2OS) cell line was assessed with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The extract significantly reduced the viability of cells in a concentration- and time-dependent manner. Cells were arrested during the S-phase of the cell cycle, and DNA damage was revealed by antibodies against histone H2AX. The apoptotic features of cell shrinkage and decrease in cell size were also observed. Western blot analysis revealed cleavage of poly (ADP-ribose)-polymerase 1 (PARP-1), in addition to increases in the proteins p53, p21, and γ-H2AX. Collectively, our findings demonstrate that the H. longiflorus extract is highly cytotoxic to U2OS cells, most likely due to its polyphenolic composition.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume70
Issue number9-10
DOIs
Publication statusPublished - Sep 1 2015

Keywords

  • Hymenocrater longiflorus
  • PARP
  • phenolic compounds
  • U2OS Cells
  • γ-H2AX

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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