Chemokine CXCL8 modulates GluR1 phosphorylation

Myriam Catalano, Flavia Trettel, Raffaela Cipriani, Clotilde Lauro, Fabrizia Sobrero, Fabrizio Eusebi, Cristina Limatola

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The chemokine interleukin 8/CXCL8 induces the phosphorylation of the GluR1 subunit of the AMPA-type glutamate receptor in neurons and transfected HEK cells, on both serine 845 (S845) and 831 (S831) residues. We previously described that CXCL8 receptor CXCR2 and GluR1 co-precipitate and that GluR1/CXCR2 co-expression both in HEK cells and neurons impairs CXCL8-induced cell migration. Here we show that replacement of S845 with Ala (A), but not with Glu (E), strongly reduces GluR1/CXCR2 interaction and abolishes the impairment of CXCL8-induced cell migration. Considered together our findings point to the phosphorylated state of S845GluR1 as a determinant of GluR1-CXCR2 physical coupling.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalJournal of Neuroimmunology
Volume198
Issue number1-2
DOIs
Publication statusPublished - Jul 31 2008

Fingerprint

Interleukin-8
Serine
Cell Movement
Interleukin-8B Receptors
Phosphorylation
Neurons
Glutamate Receptors
Chemokines
epidoxorubicin glucuronide
AMPA 1 glutamate receptor ionotropic

Keywords

  • Chemotaxis
  • CXCL8
  • Glutamate receptor
  • Phosphorylation

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Chemokine CXCL8 modulates GluR1 phosphorylation. / Catalano, Myriam; Trettel, Flavia; Cipriani, Raffaela; Lauro, Clotilde; Sobrero, Fabrizia; Eusebi, Fabrizio; Limatola, Cristina.

In: Journal of Neuroimmunology, Vol. 198, No. 1-2, 31.07.2008, p. 75-81.

Research output: Contribution to journalArticle

Catalano, M, Trettel, F, Cipriani, R, Lauro, C, Sobrero, F, Eusebi, F & Limatola, C 2008, 'Chemokine CXCL8 modulates GluR1 phosphorylation', Journal of Neuroimmunology, vol. 198, no. 1-2, pp. 75-81. https://doi.org/10.1016/j.jneuroim.2008.04.017
Catalano M, Trettel F, Cipriani R, Lauro C, Sobrero F, Eusebi F et al. Chemokine CXCL8 modulates GluR1 phosphorylation. Journal of Neuroimmunology. 2008 Jul 31;198(1-2):75-81. https://doi.org/10.1016/j.jneuroim.2008.04.017
Catalano, Myriam ; Trettel, Flavia ; Cipriani, Raffaela ; Lauro, Clotilde ; Sobrero, Fabrizia ; Eusebi, Fabrizio ; Limatola, Cristina. / Chemokine CXCL8 modulates GluR1 phosphorylation. In: Journal of Neuroimmunology. 2008 ; Vol. 198, No. 1-2. pp. 75-81.
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