Chemokine detection in the cerebral tissue of patients with posttraumatic brain contusions

Roberto Stefini, Emanuela Catenacci, Simone Piva, Silvano Sozzani, Alessandra Valerio, Riccardo Bergomi, Marco Cenzato, Pietro Mortini, Nicola Latronico

Research output: Contribution to journalArticle

Abstract

Object. The clinical outcome of patients with severe head injuries is still critically dependent on their secondary injuries. Although hypoxia and hypotension appear to mediate a substantial proportion of secondary injuries, many studies associate secondary brain injury with neuroinflammatory responses. Chemokines have been detected in the cerebrospinal fluid but not in the brain tissue of patients with head trauma. This study was performed to determine if chemokines were expressed in pericontusional brain tissue in patients with moderate or severe head trauma who underwent surgical evacuation of their brain contusions. Methods. Twelve patients with posttraumatic cerebral contusion requiring a surgical evacuation were studied. A 20- to 40-mg sample of white matter was removed from the surgical cavity in the pericontusional area. Two patients undergoing elective surgery for clip ligation of an unruptured aneurysm were used as controls. The median interval from trauma to biopsy procedure was 44 hours (range 3-360 hours). Total RNA was isolated from these samples and a ribonuclease protection assay was performed to measure the mRNA levels of several chemokines: CCL2, CCL3, CCL4, CCL5, CXCL8, CXCL10, and XCL1. Results. The CCL2, a monocyte chemoattractant produced by activated astrocytes, was the most strongly expressed chemokine, followed by CXCL8, CCL3, and CCL4. The chemokines CXCL10 and CCL5 were expressed at very low levels, and XCL1 was not detected. Conclusions. Chemokine activation occurs early after moderate or severe head trauma and is maintained for several days after trauma. This event may contribute to neuroinflammatory exacerbation of posttraumatic brain damage in the pericontusional brain tissue.

Original languageEnglish
Pages (from-to)958-962
Number of pages5
JournalJournal of Neurosurgery
Volume108
Issue number5
DOIs
Publication statusPublished - May 2008

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Keywords

  • Brain inflammation
  • Brain trauma
  • Cerebral contusion
  • Chemokine
  • Secondary brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Stefini, R., Catenacci, E., Piva, S., Sozzani, S., Valerio, A., Bergomi, R., Cenzato, M., Mortini, P., & Latronico, N. (2008). Chemokine detection in the cerebral tissue of patients with posttraumatic brain contusions. Journal of Neurosurgery, 108(5), 958-962. https://doi.org/10.3171/JNS/2008/108/5/0958