Chemokine receptor CCR5 expression in conjunctival epithelium of patients with dry eye syndrome

Objective: To characterize chemokine receptor CCR5 expression on the conjunctival epithelium in dry eye syndromes. Methods: Conjunctival impression cytology samples were obtained from normal subjects (n=15) and patients with dry eye syndrome (n=45). Cells were harvested from impression cytology samples, and flow cytometry was performed to quantitatively analyze the cell surface expression of chemokine receptor CCR5. Characterization of CCR5-positive cells was done by 2-color flow cytometry using fluorescein-conjugated anti-CCR5 and phycoerythrin-conjugated anti-CD45 antibodies (where CD45 is a marker for bone marrow-derived cells). To study CCR5 messenger RNA transcripts, real-time polymerase chain reaction was done on RNA isolated from the impression cytology samples of normal subjects (n=5) and patients with dry eye syndrome (n=14). Results: We found significant up-regulation in cell surface expression of CCR5 in patients with both aqueous tear-deficient and evaporative forms of dry eye syndrome (P