Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells

P. Lax, C. Limatola, S. Fucile, F. Trettel, S. Di Bartolomeo, M. Renzi, D. Ragozzino, F. Eusebi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Experiments were conducted in both HEK cells and cerebellar neurons to investigate whether CXC chemokine receptor 2 (CXCR2) is functionally coupled to GluR1. The co-expression of CXCR2 with GluR1 in HEK cells increased (i) the GluR1 "apparent" affinity for the transmitter; (ii) the GluR1 channel open probability; and (iii) GluR1 binding site cooperativity upon CXCR2 stimulation with CXC chemokine ligand 2 (CXCL2). The affinity of C-terminal-deleted GluR1 for glutamate (Glu) remained stable instead. Furthermore, CXCL2 increased the binding site cooperativity of AMPA receptors in rat cerebellar granule cells; and the amplitude of spontaneous excitatory postsynaptic current (sEPSCs) in Purkinje neurons (PNs). Our findings indicate that the coupling of CXCR2 with GluR1 may modulate glutamatergic synaptic transmission.

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalJournal of Neuroimmunology
Volume129
Issue number1-2
DOIs
Publication statusPublished - 2002

Fingerprint

Interleukin-8B Receptors
AMPA Receptors
Chemokine Receptors
Glutamate Receptors
CXC Chemokines
Binding Sites
Ligands
Excitatory Postsynaptic Potentials
Purkinje Cells
Synaptic Transmission
Glutamic Acid
Neurons

Keywords

  • AMPA
  • Cerebellar granule cells
  • CXCL2
  • GluR1 binding affinity
  • HEK cells
  • Purkinje neurons

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells. / Lax, P.; Limatola, C.; Fucile, S.; Trettel, F.; Di Bartolomeo, S.; Renzi, M.; Ragozzino, D.; Eusebi, F.

In: Journal of Neuroimmunology, Vol. 129, No. 1-2, 2002, p. 66-73.

Research output: Contribution to journalArticle

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AU - Lax, P.

AU - Limatola, C.

AU - Fucile, S.

AU - Trettel, F.

AU - Di Bartolomeo, S.

AU - Renzi, M.

AU - Ragozzino, D.

AU - Eusebi, F.

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