Chemokine receptor interactions with virus-like particles

Luigi Buonaguro, Maria Tagliamonte, Maria Luisa Visciano

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Virus-like particles (VLPs) presenting conformational envelope proteins on their surface represent an invaluable tool to study molecular interactions between viruses and cellular receptors/co-receptors, eliminating biological risks associated with working with live native viruses. The availability of target cells expressing speci fi c chemokine receptors facilitates the dissection of speci fi c interactions between humanimmunodeficiency virus (HIV) viral envelope proteins and these receptors in the laboratory. Here, we describe a method to evaluate HIV-VLP binding to cellular chemokine co-receptors, by carboxy fluorescein succinimidyl ester labeling and cellular uptake.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages57-66
Number of pages10
Volume1013
ISBN (Print)9781627034258
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1013
ISSN (Print)10643745

Keywords

  • Cell binding
  • CFSE labeling
  • Chemokine receptors
  • Human immunode ficiency virus
  • Virus-like particles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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