Abstract
Bronchiolitis obliterans syndrome (BOS) is one of the most important factors limiting the long-term survival of lung transplant recipients (LTR), however its pathogenesis still remains unclear. We hypothesized that an increased production of certain specific proinflammatory mediators in the first post-transplant year would predispose to BOS. We retrospectively evaluated temporal kinetics of some CC chemokines that have not yet been evaluated, including CCL3/MIP1-α, CCL4/MIP1-β, CCL17/TARC, CCL19/MIP3-β, CCL20/MIP3-α, CCL22/MDC and CCL26/eotaxin, in broncho-alveolar lavage fluid (BAL-f) in the first post-transplant year in a cohort of 8 LTR before the development of BOS (pre-BOS LTR) and 8 LTR with long-term stable clinical conditions (stable LTR). Chemokine levels were assayed by means of a multiplex sandwich ELISA. Furthermore, for those ligands which resulted significantly predictive of BOS onset, we analyzed the expression of specific receptors (CCR) on BAL cells. The proportion of CCR-expressing BAL cells was assessed by flow cytometry. We demonstrated that MIP3-β/CCL19, MIP3-α/CCL20, MDC/CCL22 levels at 6 months post-transplant significantly predicted BOS onset. In addition, the temporal behavior of these factors resulted significantly different in pre-BOS patients as compared to stable LTR. Finally the expression of CCR was documented on BAL lymphocytes and macrophages, and, in some cases, their expression was found to vary between the two groups. Within the complexity of the chemokine network, these three CCL factors could play an additive role in the pathogenesis of the inflammatory process leading to bronchiolar fibro-obliteration.
| Original language | English |
|---|---|
| Pages (from-to) | 275-280 |
| Number of pages | 6 |
| Journal | Transplant Immunology |
| Volume | 18 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 2008 |
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Keywords
- CCL19/CCR7
- CCL20/CCR6
- CCL22/CCR4
- Chronic lung rejection
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Transplantation
Cite this
Chemokine redundancy in BOS pathogenesis. A possible role also for the CC chemokines : MIP3-beta, MIP3-alpha, MDC and their specific receptors. / Meloni, F.; Solari, N.; Miserere, S.; Morosini, M.; Cascina, A.; Klersy, C.; Arbustini, E.; Pellegrini, C.; Viganò, M.; Fietta, A. M.
In: Transplant Immunology, Vol. 18, No. 3, 01.2008, p. 275-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemokine redundancy in BOS pathogenesis. A possible role also for the CC chemokines
T2 - MIP3-beta, MIP3-alpha, MDC and their specific receptors
AU - Meloni, F.
AU - Solari, N.
AU - Miserere, S.
AU - Morosini, M.
AU - Cascina, A.
AU - Klersy, C.
AU - Arbustini, E.
AU - Pellegrini, C.
AU - Viganò, M.
AU - Fietta, A. M.
PY - 2008/1
Y1 - 2008/1
N2 - Bronchiolitis obliterans syndrome (BOS) is one of the most important factors limiting the long-term survival of lung transplant recipients (LTR), however its pathogenesis still remains unclear. We hypothesized that an increased production of certain specific proinflammatory mediators in the first post-transplant year would predispose to BOS. We retrospectively evaluated temporal kinetics of some CC chemokines that have not yet been evaluated, including CCL3/MIP1-α, CCL4/MIP1-β, CCL17/TARC, CCL19/MIP3-β, CCL20/MIP3-α, CCL22/MDC and CCL26/eotaxin, in broncho-alveolar lavage fluid (BAL-f) in the first post-transplant year in a cohort of 8 LTR before the development of BOS (pre-BOS LTR) and 8 LTR with long-term stable clinical conditions (stable LTR). Chemokine levels were assayed by means of a multiplex sandwich ELISA. Furthermore, for those ligands which resulted significantly predictive of BOS onset, we analyzed the expression of specific receptors (CCR) on BAL cells. The proportion of CCR-expressing BAL cells was assessed by flow cytometry. We demonstrated that MIP3-β/CCL19, MIP3-α/CCL20, MDC/CCL22 levels at 6 months post-transplant significantly predicted BOS onset. In addition, the temporal behavior of these factors resulted significantly different in pre-BOS patients as compared to stable LTR. Finally the expression of CCR was documented on BAL lymphocytes and macrophages, and, in some cases, their expression was found to vary between the two groups. Within the complexity of the chemokine network, these three CCL factors could play an additive role in the pathogenesis of the inflammatory process leading to bronchiolar fibro-obliteration.
AB - Bronchiolitis obliterans syndrome (BOS) is one of the most important factors limiting the long-term survival of lung transplant recipients (LTR), however its pathogenesis still remains unclear. We hypothesized that an increased production of certain specific proinflammatory mediators in the first post-transplant year would predispose to BOS. We retrospectively evaluated temporal kinetics of some CC chemokines that have not yet been evaluated, including CCL3/MIP1-α, CCL4/MIP1-β, CCL17/TARC, CCL19/MIP3-β, CCL20/MIP3-α, CCL22/MDC and CCL26/eotaxin, in broncho-alveolar lavage fluid (BAL-f) in the first post-transplant year in a cohort of 8 LTR before the development of BOS (pre-BOS LTR) and 8 LTR with long-term stable clinical conditions (stable LTR). Chemokine levels were assayed by means of a multiplex sandwich ELISA. Furthermore, for those ligands which resulted significantly predictive of BOS onset, we analyzed the expression of specific receptors (CCR) on BAL cells. The proportion of CCR-expressing BAL cells was assessed by flow cytometry. We demonstrated that MIP3-β/CCL19, MIP3-α/CCL20, MDC/CCL22 levels at 6 months post-transplant significantly predicted BOS onset. In addition, the temporal behavior of these factors resulted significantly different in pre-BOS patients as compared to stable LTR. Finally the expression of CCR was documented on BAL lymphocytes and macrophages, and, in some cases, their expression was found to vary between the two groups. Within the complexity of the chemokine network, these three CCL factors could play an additive role in the pathogenesis of the inflammatory process leading to bronchiolar fibro-obliteration.
KW - CCL19/CCR7
KW - CCL20/CCR6
KW - CCL22/CCR4
KW - Chronic lung rejection
UR - http://www.scopus.com/inward/record.url?scp=36349024682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36349024682&partnerID=8YFLogxK
U2 - 10.1016/j.trim.2007.08.004
DO - 10.1016/j.trim.2007.08.004
M3 - Article
C2 - 18047937
AN - SCOPUS:36349024682
VL - 18
SP - 275
EP - 280
JO - Transplant Immunology
JF - Transplant Immunology
SN - 0966-3274
IS - 3
ER -