Chemosensitivity of human tumor clonogenic cells simultaneously assayed in agar diffusion chambers and in a two-layer agar culture system

A. F. Sobrero, J. C. Marsh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have compared plating efficiencies (PE) of 20 fresh and cryopreserved samples of human solid tumors, disaggregated and cloned simultaneously in a two-layer agar culture system (2LACS) and in agar diffusion chambers (ADC). A significant correlation (0.001 <P <0.01, paired t test) was found between PE, confirming that the two methods reflect the same property of tumor cells: their clonogenic potential. The median PE in ADC was 1.4 and 3.3 times higher than that in the 2LACS for cryopreserved and fresh specimens, respectively. One- and two-drug doses, approximating clinically achievable concentrations, were assayed simultaneously in the ADC and in the 2LACS, respectively, on 15 tumor specimens. In this way we evaluated the effects of eight drugs, two to five for each tumor, in a static (2LACS) versus a dynamic (ADC) system. The comparison of percent survival in ADC versus that in the 2LACS at both concentrations tested showed no statistically significant rank correlation. If the data regarding cyclophosphamide and mitomycin, which produced significant cell kill in the ADC and almost none in the 2LACS, were excluded, the rank correlation was still not significant. We conclude that the widely used 2LACS is unsuitable to study cyclophosphamide and mitomycin, for which the ADC technique may be a valid alternative.

Original languageEnglish
Pages (from-to)615-624
Number of pages10
JournalCancer Treatment Reports
Volume68
Issue number4
Publication statusPublished - 1984

Fingerprint

Agar
Neoplasms
Mitomycin
Cyclophosphamide
Pharmaceutical Preparations
Survival

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

@article{a31f36472a4c4ac38a83dc7daa751304,
title = "Chemosensitivity of human tumor clonogenic cells simultaneously assayed in agar diffusion chambers and in a two-layer agar culture system",
abstract = "We have compared plating efficiencies (PE) of 20 fresh and cryopreserved samples of human solid tumors, disaggregated and cloned simultaneously in a two-layer agar culture system (2LACS) and in agar diffusion chambers (ADC). A significant correlation (0.001 <P <0.01, paired t test) was found between PE, confirming that the two methods reflect the same property of tumor cells: their clonogenic potential. The median PE in ADC was 1.4 and 3.3 times higher than that in the 2LACS for cryopreserved and fresh specimens, respectively. One- and two-drug doses, approximating clinically achievable concentrations, were assayed simultaneously in the ADC and in the 2LACS, respectively, on 15 tumor specimens. In this way we evaluated the effects of eight drugs, two to five for each tumor, in a static (2LACS) versus a dynamic (ADC) system. The comparison of percent survival in ADC versus that in the 2LACS at both concentrations tested showed no statistically significant rank correlation. If the data regarding cyclophosphamide and mitomycin, which produced significant cell kill in the ADC and almost none in the 2LACS, were excluded, the rank correlation was still not significant. We conclude that the widely used 2LACS is unsuitable to study cyclophosphamide and mitomycin, for which the ADC technique may be a valid alternative.",
author = "Sobrero, {A. F.} and Marsh, {J. C.}",
year = "1984",
language = "English",
volume = "68",
pages = "615--624",
journal = "Cancer Treatment Reports",
issn = "0361-5960",
publisher = "U.S. National Cancer Institute",
number = "4",

}

TY - JOUR

T1 - Chemosensitivity of human tumor clonogenic cells simultaneously assayed in agar diffusion chambers and in a two-layer agar culture system

AU - Sobrero, A. F.

AU - Marsh, J. C.

PY - 1984

Y1 - 1984

N2 - We have compared plating efficiencies (PE) of 20 fresh and cryopreserved samples of human solid tumors, disaggregated and cloned simultaneously in a two-layer agar culture system (2LACS) and in agar diffusion chambers (ADC). A significant correlation (0.001 <P <0.01, paired t test) was found between PE, confirming that the two methods reflect the same property of tumor cells: their clonogenic potential. The median PE in ADC was 1.4 and 3.3 times higher than that in the 2LACS for cryopreserved and fresh specimens, respectively. One- and two-drug doses, approximating clinically achievable concentrations, were assayed simultaneously in the ADC and in the 2LACS, respectively, on 15 tumor specimens. In this way we evaluated the effects of eight drugs, two to five for each tumor, in a static (2LACS) versus a dynamic (ADC) system. The comparison of percent survival in ADC versus that in the 2LACS at both concentrations tested showed no statistically significant rank correlation. If the data regarding cyclophosphamide and mitomycin, which produced significant cell kill in the ADC and almost none in the 2LACS, were excluded, the rank correlation was still not significant. We conclude that the widely used 2LACS is unsuitable to study cyclophosphamide and mitomycin, for which the ADC technique may be a valid alternative.

AB - We have compared plating efficiencies (PE) of 20 fresh and cryopreserved samples of human solid tumors, disaggregated and cloned simultaneously in a two-layer agar culture system (2LACS) and in agar diffusion chambers (ADC). A significant correlation (0.001 <P <0.01, paired t test) was found between PE, confirming that the two methods reflect the same property of tumor cells: their clonogenic potential. The median PE in ADC was 1.4 and 3.3 times higher than that in the 2LACS for cryopreserved and fresh specimens, respectively. One- and two-drug doses, approximating clinically achievable concentrations, were assayed simultaneously in the ADC and in the 2LACS, respectively, on 15 tumor specimens. In this way we evaluated the effects of eight drugs, two to five for each tumor, in a static (2LACS) versus a dynamic (ADC) system. The comparison of percent survival in ADC versus that in the 2LACS at both concentrations tested showed no statistically significant rank correlation. If the data regarding cyclophosphamide and mitomycin, which produced significant cell kill in the ADC and almost none in the 2LACS, were excluded, the rank correlation was still not significant. We conclude that the widely used 2LACS is unsuitable to study cyclophosphamide and mitomycin, for which the ADC technique may be a valid alternative.

UR - http://www.scopus.com/inward/record.url?scp=0021344127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021344127&partnerID=8YFLogxK

M3 - Article

VL - 68

SP - 615

EP - 624

JO - Cancer Treatment Reports

JF - Cancer Treatment Reports

SN - 0361-5960

IS - 4

ER -