Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells

Anna Corcione, Giuseppe Tortolina, Raffaella Bonecchi, Nicoletta Battilana, Giuseppe Taborelli, Fabio Malavasi, Silvano Sozzani, Luciano Ottonello, Franco Dallegri, Vito Pistoia

Research output: Contribution to journalArticle

Abstract

We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.

Original languageEnglish
Pages (from-to)883-892
Number of pages10
JournalInternational Immunology
Volume14
Issue number8
Publication statusPublished - 2002

Fingerprint

CCR1 Receptors
Macrophage Inflammatory Proteins
Palatine Tonsil
Chemotaxis
B-Lymphocytes
Ligands
Chemokine CCL22
Chemokine CCL8
Chemokine CCL7
Chemokine CCL2
B-Lymphocyte Subsets
Germinal Center
Locomotion
Chemokines
CC Chemokines
Cell Movement

Keywords

  • B cells subsets
  • Chemokine receptors
  • Chemokines
  • Locomotion

ASJC Scopus subject areas

  • Immunology

Cite this

Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells. / Corcione, Anna; Tortolina, Giuseppe; Bonecchi, Raffaella; Battilana, Nicoletta; Taborelli, Giuseppe; Malavasi, Fabio; Sozzani, Silvano; Ottonello, Luciano; Dallegri, Franco; Pistoia, Vito.

In: International Immunology, Vol. 14, No. 8, 2002, p. 883-892.

Research output: Contribution to journalArticle

Corcione, A, Tortolina, G, Bonecchi, R, Battilana, N, Taborelli, G, Malavasi, F, Sozzani, S, Ottonello, L, Dallegri, F & Pistoia, V 2002, 'Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells', International Immunology, vol. 14, no. 8, pp. 883-892.
Corcione, Anna ; Tortolina, Giuseppe ; Bonecchi, Raffaella ; Battilana, Nicoletta ; Taborelli, Giuseppe ; Malavasi, Fabio ; Sozzani, Silvano ; Ottonello, Luciano ; Dallegri, Franco ; Pistoia, Vito. / Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells. In: International Immunology. 2002 ; Vol. 14, No. 8. pp. 883-892.
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T1 - Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells

AU - Corcione, Anna

AU - Tortolina, Giuseppe

AU - Bonecchi, Raffaella

AU - Battilana, Nicoletta

AU - Taborelli, Giuseppe

AU - Malavasi, Fabio

AU - Sozzani, Silvano

AU - Ottonello, Luciano

AU - Dallegri, Franco

AU - Pistoia, Vito

PY - 2002

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N2 - We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.

AB - We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.

KW - B cells subsets

KW - Chemokine receptors

KW - Chemokines

KW - Locomotion

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