Abstract
We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.
| Original language | English |
|---|---|
| Pages (from-to) | 883-892 |
| Number of pages | 10 |
| Journal | International Immunology |
| Volume | 14 |
| Issue number | 8 |
| Publication status | Published - 2002 |
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Keywords
- B cells subsets
- Chemokine receptors
- Chemokines
- Locomotion
ASJC Scopus subject areas
- Immunology
Cite this
Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells. / Corcione, Anna; Tortolina, Giuseppe; Bonecchi, Raffaella; Battilana, Nicoletta; Taborelli, Giuseppe; Malavasi, Fabio; Sozzani, Silvano; Ottonello, Luciano; Dallegri, Franco; Pistoia, Vito.
In: International Immunology, Vol. 14, No. 8, 2002, p. 883-892.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemotaxis of human tonsil B lymphocytes to CC chemokine receptor (CCR) 1, CCR2 and CCR4 ligands is restricted to non-germinal center cells
AU - Corcione, Anna
AU - Tortolina, Giuseppe
AU - Bonecchi, Raffaella
AU - Battilana, Nicoletta
AU - Taborelli, Giuseppe
AU - Malavasi, Fabio
AU - Sozzani, Silvano
AU - Ottonello, Luciano
AU - Dallegri, Franco
AU - Pistoia, Vito
PY - 2002
Y1 - 2002
N2 - We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.
AB - We have investigated the effects of nine CC chemokines, i.e. macrophage inflammatory protein (MIP)-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20, MIP-5/CCL15, monocyte chemotactic protein (MCP)-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, eotaxin/CCL11 and macrophage-derived chemokine (MDC)/ CCL22 on the locomotion of human tonsil B lymphocytes and their subsets. Upon isolation, B cells were poorly responsive, but, following short-term culture, they displayed statistically significant chemotactic responses (P <0.001) to MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC. CC chemokine receptor (CCR) 1 to CCR6 were up-regulated after culture, MIP-1β, MIP-3α and eotaxin did not stimulate B cell migration. Scattered information is available on B cell subset responses to chemokines. Therefore, we investigated the effects of MIP-1α, MIP-5, MCP-1, MCP-2, MCP-3 and MDC on the in vitro locomotion of non-germinal center (GC) (CD38-) and GC (CD38+) B cells. All chemokines enhanced significantly (P <0.001) the migration of the former, but not of the latter, cells. CCR1, CCR2 and CCR4 were detected by flow cytometry on non-GC (i.e. naive and memory) B cells, whereas they were absent (CCR1 and CCR2) or poorly expressed (CCR4) on GC B cells.
KW - B cells subsets
KW - Chemokine receptors
KW - Chemokines
KW - Locomotion
UR - http://www.scopus.com/inward/record.url?scp=0035993992&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035993992&partnerID=8YFLogxK
M3 - Article
C2 - 12147625
AN - SCOPUS:0035993992
VL - 14
SP - 883
EP - 892
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 8
ER -