Abstract
The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. The CAM may also be used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an antiangiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 47-57 |
| Number of pages | 11 |
| Volume | 843 |
| DOIs | |
| Publication status | Published - 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 843 |
| ISSN (Print) | 10643745 |
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Keywords
- Angiogenesis
- Antiangiogenesis
- Chorioallantoic membrane
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Chicken chorioallantoic membrane angiogenesis model. / Ribatti, Domenico.
Methods in Molecular Biology. Vol. 843 2012. p. 47-57 (Methods in Molecular Biology; Vol. 843).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Chicken chorioallantoic membrane angiogenesis model
AU - Ribatti, Domenico
PY - 2012
Y1 - 2012
N2 - The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. The CAM may also be used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an antiangiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.
AB - The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. The CAM may also be used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an antiangiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.
KW - Angiogenesis
KW - Antiangiogenesis
KW - Chorioallantoic membrane
UR - http://www.scopus.com/inward/record.url?scp=84856283151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856283151&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-523-7_5
DO - 10.1007/978-1-61779-523-7_5
M3 - Chapter
C2 - 22222520
AN - SCOPUS:84856283151
SN - 9781617795220
VL - 843
T3 - Methods in Molecular Biology
SP - 47
EP - 57
BT - Methods in Molecular Biology
ER -