Chikungunya virus isolates with/without A226V mutation show different sensitivity to IFN-α, but similar replication kinetics in non human primate cells

Licia Bordi; Silvia Meschi; Marina Selleri; Eleonora Lalle; Concetta Castilletti; Fabrizio Carletti; Roberta Chiappini; Antonino Di Caro; Maria Rosaria Capobianchi

Chikungunya virus isolates with/without A226V mutation show different sensitivity to IFN-α, but similar replication kinetics in non human primate cells

Licia Bordi, Silvia Meschi, Marina Selleri, Eleonora Lalle, Concetta Castilletti, Fabrizio Carletti, Roberta Chiappini, Antonino Di Caro, Maria Rosaria Capobianchi

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for the first autochthonous Italian outbreak in 2007. A226V mutation in El has been associated with enhanced replication in A. albopictus vector. Possible involvement of this mutation in enhanced infection capability in primate cells and sensitivity to exogenous interferon (IFN)-α was investigated. No significant differences were observed between the two isolates in terms of replication kinetic, virus yield and cy-topathic effect (CPE). Interestingly, the A226V-carrying strain was more susceptible to the antiviral action of recombinant IFN-α. The interplay between A226V mutation and innate defence mechanisms needs further investigation.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	New Microbiologica
Volume	34
Issue number	1
Publication status	Published - Jan 2011

Keywords

A226V mutation
Chikungunya
Interferon α
Pathogenesis

ASJC Scopus subject areas

Microbiology (medical)

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Chikungunya virus isolates with/without A226V mutation show different sensitivity to IFN-α, but similar replication kinetics in non human primate cells. / Bordi, Licia ; Meschi, Silvia ; Selleri, Marina ; Lalle, Eleonora ; Castilletti, Concetta ; Carletti, Fabrizio ; Chiappini, Roberta ; Di Caro, Antonino ; Rosaria Capobianchi, Maria.

In: New Microbiologica, Vol. 34, No. 1, 01.2011, p. 87-91.

Research output: Contribution to journal › Article › peer-review

Bordi, Licia ; Meschi, Silvia ; Selleri, Marina ; Lalle, Eleonora ; Castilletti, Concetta ; Carletti, Fabrizio ; Chiappini, Roberta ; Di Caro, Antonino ; Rosaria Capobianchi, Maria. / Chikungunya virus isolates with/without A226V mutation show different sensitivity to IFN-α, but similar replication kinetics in non human primate cells. In: New Microbiologica. 2011 ; Vol. 34, No. 1. pp. 87-91.

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abstract = "Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for the first autochthonous Italian outbreak in 2007. A226V mutation in El has been associated with enhanced replication in A. albopictus vector. Possible involvement of this mutation in enhanced infection capability in primate cells and sensitivity to exogenous interferon (IFN)-α was investigated. No significant differences were observed between the two isolates in terms of replication kinetic, virus yield and cy-topathic effect (CPE). Interestingly, the A226V-carrying strain was more susceptible to the antiviral action of recombinant IFN-α. The interplay between A226V mutation and innate defence mechanisms needs further investigation.",

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AU - Selleri, Marina

AU - Lalle, Eleonora

AU - Castilletti, Concetta

AU - Carletti, Fabrizio

AU - Chiappini, Roberta

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