TY - JOUR
T1 - Children on continuous ambulatorial peritoneal dialysis
T2 - Muscle and plasma proteins, amino acids and nutritional status
AU - Canepa, A.
AU - Divino Filho, J. C.
AU - Forsberg, A. M.
AU - Perfumo, F.
AU - Carrea, A.
AU - Verrina, E.
AU - Podestá, E.
AU - Gusmano, R.
AU - Bergström, J.
PY - 1996
Y1 - 1996
N2 - The aim of the study was to investigate plasma and muscle amino acid (AA) levels in children on continuous ambulatory peritoneal dialysis (CAPD) and their relationship to various indices of nutritional status. Ten children with a mean age of 6.4 ± 5.6 yrs were evaluated. Muscle biopsies and venous blood samples were taken after an overnight fast. Muscle samples were obtained from rectus abdominis. Data were compared with those of a control group of 22 children who were undergoing elective surgery. Informed consent was obtained from the parents. The plasma concentration of most of the essential AA (valine, leucine, isoleucine, lysine, methionine and tyrosine) were significantly reduced and the levels of some non essential AA (aspartic acid, glycine, citrulline, 1-3 methihystidine, taurine + alanine) were significantly higher than in the controls. Muscle intracellular free essential AA concentrations, except the low levels of valine and leucine did not differ significantly from values in the controls. Among non essential AA, aspartic acid, glutamic acid and ornitine showed significantly increased intracellular concentrations. No significant correlations were found between plasma and muscle AA concentration and ASP(alkali-soluble protein)/DNA ratio, serum albumin, transferrin, bicarbonate levels and duration of CAPD. Instead, a significant correlation was noted between the muscle ASP/DNA ratio, an indicator of the amount of cell proteins per cell unit, and age (r = 0.714, p <0.05). Muscle Branched chain AA levels were significantly correlated to body mass index (BMI) (r = 0.648, p <0.05).
AB - The aim of the study was to investigate plasma and muscle amino acid (AA) levels in children on continuous ambulatory peritoneal dialysis (CAPD) and their relationship to various indices of nutritional status. Ten children with a mean age of 6.4 ± 5.6 yrs were evaluated. Muscle biopsies and venous blood samples were taken after an overnight fast. Muscle samples were obtained from rectus abdominis. Data were compared with those of a control group of 22 children who were undergoing elective surgery. Informed consent was obtained from the parents. The plasma concentration of most of the essential AA (valine, leucine, isoleucine, lysine, methionine and tyrosine) were significantly reduced and the levels of some non essential AA (aspartic acid, glycine, citrulline, 1-3 methihystidine, taurine + alanine) were significantly higher than in the controls. Muscle intracellular free essential AA concentrations, except the low levels of valine and leucine did not differ significantly from values in the controls. Among non essential AA, aspartic acid, glutamic acid and ornitine showed significantly increased intracellular concentrations. No significant correlations were found between plasma and muscle AA concentration and ASP(alkali-soluble protein)/DNA ratio, serum albumin, transferrin, bicarbonate levels and duration of CAPD. Instead, a significant correlation was noted between the muscle ASP/DNA ratio, an indicator of the amount of cell proteins per cell unit, and age (r = 0.714, p <0.05). Muscle Branched chain AA levels were significantly correlated to body mass index (BMI) (r = 0.648, p <0.05).
KW - ASP/DNA
KW - Children
KW - Continuous ambulatory peritoneal dialysis
KW - Muscle-Plasma-Amino acids
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M3 - Article
C2 - 8869790
AN - SCOPUS:9444262439
VL - 46
SP - 125
EP - 131
JO - Clinical Nephrology
JF - Clinical Nephrology
SN - 0301-0430
IS - 2
ER -