Chimeric human epidermal reconstructs to study the role of melanocytes and keratinocytes in pigmentation and photoprotection

Sandrine Bessou-Touya, Mauro Picardo, Vittoria Maresca, Jean Etienne Surlève-Bazeille, Catherine Pain, Alain Taïeb

Research output: Contribution to journalArticle

Abstract

Chimeric epidermal reconstructs made with Negroid melanocytes and Caucasoid keratinocytes (or vice versa) were studied before and after UVB irradiation to understand the respective roles of these cells in tanning and photoprotection, especially lipoperoxidation and enzymatic defences against free radicals. Using this approach, we have confirmed overall the theory of the epidermal melanin unit. We have also shown that melanocytes of poorly tanning Caucasoids, which have a comparatively higher content of unsaturated fatty acids in their cell membrane, are more prone to the peroxidative effects of UV light, and that keratinocytes participate in photoprotection via phototype-dependent antioxidant enzyme activities, especially for catalase.

Original languageEnglish
Pages (from-to)1103-1108
Number of pages6
JournalJournal of Investigative Dermatology
Volume111
Issue number6
DOIs
Publication statusPublished - 1998

Keywords

  • Catalase
  • Epidermal lipids
  • Melanin
  • Superoxide dismutase

ASJC Scopus subject areas

  • Dermatology

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