Chlamydia pneumoniae and chronic bronchitis: Association with severity and bacterial clearance following treatment

Francesco Blasi, S. Damato, R. Cosentini, P. Tarsia, R. Raccanelli, S. Centanni, L. Allegra, C. Arosio, C. Dotti, L. Fagetti, M. Forloni, A. Grugnetti, D. Lattuada, N. Morelli, C. Papetti, C. Pravettoni, G. Graziadei, G. G. Torgano, C. Canetta, P. A. Santus

Research output: Contribution to journalArticlepeer-review

Abstract

Background: A study was undertaken to evaluate Chlamydia pneumoniae chronic infection, other respiratory infections, and functional impairment in patients with chronic bronchitis (stage 1) and to examine chronic C pneumoniae infection, rate of acute exacerbations of chronic bronchitis, and rate of C pneumoniae eradication following antibiotic treatment (stage 2). Methods: In the stage 1 study respiratory specimens from 42 patients with steady state chronic bronchitis were analysed for Gram staining, sputum culture, and C pneumoniae DNA detection by nested touchdown polymerase chain reaction (PCR). On the basis of the results of stage 1, a second population of 141 consecutive patients with steady state mild to moderate chronic bronchitis (FEV1 ≥50% predicted) was studied. On admission, at regular intervals, and at exacerbation all patients underwent serological testing for C pneumoniae (microimmunofluorescence) and a nested touchdown PCR to detect C pneumoniae DNA was performed on peripheral blood mononuclear cells (PBMCs). Patients were assessed over a 12 month period. Information regarding the previous 12 months was taken from medical records. Results: Chronic colonisation of the sputum with C pneumoniae was significantly associated with lower FEV1 and greater airway bacterial colonisation. On admission to the stage 2 study, 80 patients were PCR negative and 61 were PCR positive. Over the 2 years a mean (SD) of 1.43 (1.32) acute exacerbations occurred in PCR negative patients and 2.03 (1.21) in PCR positive patients (p

Original languageEnglish
Pages (from-to)672-676
Number of pages5
JournalThorax
Volume57
Issue number8
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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