Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection

Francesco Blasi, Jens Boman, Gloria Esposito, Germano Melissano, Roberto Chiesa, Roberto Cosentini, Paolo Tarsia, Yamume Tshomba, Monia Betti, Marinella Alessi, Nicoletta Morelli, Luigi Allegra

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Abdominal aortic aneurysm tissue and peripheral blood mononuclear cells (PBMC) of 41 consecutive subjects undergoing abdominal aortic aneurysm surgery were analyzed by polymerase chain reaction (PCR) for the presence of Chlamydia pneumoniae, Mycoplasma pneumoniae, and Helicobacter pylori DNA. Twenty patients (49%) were positive for C. pneumoniae DNA-16 (39%) in both PBMC and aneurysm tissue, 3 (7.3%) in PBMC only, and 1 (2.4%) in the artery specimen only. Previous exposure to C. pneumoniae was confirmed in 19 (95%) of the 20 PCR positive subjects by C. pneumoniae-specific serology, using the microimmunofluorescence test. None was positive for H. pylori or M. pneumoniae DNA, either in the PBMC or in the artery specimens. In conclusion, carriage of C. pneumoniae DNA is common both in PBMC and in abdominal aortic tissue from patients undergoing abdominal aneurysm surgery. Blood PCR may be a useful tool for identifying subjects carrying C. pneumoniae in the vascular wall.

Original languageEnglish
Pages (from-to)2074-2076
Number of pages3
JournalJournal of Infectious Diseases
Volume180
Issue number6
DOIs
Publication statusPublished - 1999

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Chlamydophila pneumoniae
Blood Vessels
Blood Cells
DNA
Infection
Mycoplasma pneumoniae
Abdominal Aortic Aneurysm
Helicobacter pylori
Polymerase Chain Reaction
Aneurysm
Arteries
Serology

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection. / Blasi, Francesco; Boman, Jens; Esposito, Gloria; Melissano, Germano; Chiesa, Roberto; Cosentini, Roberto; Tarsia, Paolo; Tshomba, Yamume; Betti, Monia; Alessi, Marinella; Morelli, Nicoletta; Allegra, Luigi.

In: Journal of Infectious Diseases, Vol. 180, No. 6, 1999, p. 2074-2076.

Research output: Contribution to journalArticle

Blasi, Francesco ; Boman, Jens ; Esposito, Gloria ; Melissano, Germano ; Chiesa, Roberto ; Cosentini, Roberto ; Tarsia, Paolo ; Tshomba, Yamume ; Betti, Monia ; Alessi, Marinella ; Morelli, Nicoletta ; Allegra, Luigi. / Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection. In: Journal of Infectious Diseases. 1999 ; Vol. 180, No. 6. pp. 2074-2076.
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AU - Chiesa, Roberto

AU - Cosentini, Roberto

AU - Tarsia, Paolo

AU - Tshomba, Yamume

AU - Betti, Monia

AU - Alessi, Marinella

AU - Morelli, Nicoletta

AU - Allegra, Luigi

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