Abstract
Abdominal aortic aneurysm tissue and peripheral blood mononuclear cells (PBMC) of 41 consecutive subjects undergoing abdominal aortic aneurysm surgery were analyzed by polymerase chain reaction (PCR) for the presence of Chlamydia pneumoniae, Mycoplasma pneumoniae, and Helicobacter pylori DNA. Twenty patients (49%) were positive for C. pneumoniae DNA-16 (39%) in both PBMC and aneurysm tissue, 3 (7.3%) in PBMC only, and 1 (2.4%) in the artery specimen only. Previous exposure to C. pneumoniae was confirmed in 19 (95%) of the 20 PCR positive subjects by C. pneumoniae-specific serology, using the microimmunofluorescence test. None was positive for H. pylori or M. pneumoniae DNA, either in the PBMC or in the artery specimens. In conclusion, carriage of C. pneumoniae DNA is common both in PBMC and in abdominal aortic tissue from patients undergoing abdominal aneurysm surgery. Blood PCR may be a useful tool for identifying subjects carrying C. pneumoniae in the vascular wall.
| Original language | English |
|---|---|
| Pages (from-to) | 2074-2076 |
| Number of pages | 3 |
| Journal | Journal of Infectious Diseases |
| Volume | 180 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1999 |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
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Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection. / Blasi, Francesco; Boman, Jens; Esposito, Gloria; Melissano, Germano; Chiesa, Roberto; Cosentini, Roberto; Tarsia, Paolo; Tshomba, Yamume; Betti, Monia; Alessi, Marinella; Morelli, Nicoletta; Allegra, Luigi.
In: Journal of Infectious Diseases, Vol. 180, No. 6, 1999, p. 2074-2076.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells is predictive of vascular infection
AU - Blasi, Francesco
AU - Boman, Jens
AU - Esposito, Gloria
AU - Melissano, Germano
AU - Chiesa, Roberto
AU - Cosentini, Roberto
AU - Tarsia, Paolo
AU - Tshomba, Yamume
AU - Betti, Monia
AU - Alessi, Marinella
AU - Morelli, Nicoletta
AU - Allegra, Luigi
PY - 1999
Y1 - 1999
N2 - Abdominal aortic aneurysm tissue and peripheral blood mononuclear cells (PBMC) of 41 consecutive subjects undergoing abdominal aortic aneurysm surgery were analyzed by polymerase chain reaction (PCR) for the presence of Chlamydia pneumoniae, Mycoplasma pneumoniae, and Helicobacter pylori DNA. Twenty patients (49%) were positive for C. pneumoniae DNA-16 (39%) in both PBMC and aneurysm tissue, 3 (7.3%) in PBMC only, and 1 (2.4%) in the artery specimen only. Previous exposure to C. pneumoniae was confirmed in 19 (95%) of the 20 PCR positive subjects by C. pneumoniae-specific serology, using the microimmunofluorescence test. None was positive for H. pylori or M. pneumoniae DNA, either in the PBMC or in the artery specimens. In conclusion, carriage of C. pneumoniae DNA is common both in PBMC and in abdominal aortic tissue from patients undergoing abdominal aneurysm surgery. Blood PCR may be a useful tool for identifying subjects carrying C. pneumoniae in the vascular wall.
AB - Abdominal aortic aneurysm tissue and peripheral blood mononuclear cells (PBMC) of 41 consecutive subjects undergoing abdominal aortic aneurysm surgery were analyzed by polymerase chain reaction (PCR) for the presence of Chlamydia pneumoniae, Mycoplasma pneumoniae, and Helicobacter pylori DNA. Twenty patients (49%) were positive for C. pneumoniae DNA-16 (39%) in both PBMC and aneurysm tissue, 3 (7.3%) in PBMC only, and 1 (2.4%) in the artery specimen only. Previous exposure to C. pneumoniae was confirmed in 19 (95%) of the 20 PCR positive subjects by C. pneumoniae-specific serology, using the microimmunofluorescence test. None was positive for H. pylori or M. pneumoniae DNA, either in the PBMC or in the artery specimens. In conclusion, carriage of C. pneumoniae DNA is common both in PBMC and in abdominal aortic tissue from patients undergoing abdominal aneurysm surgery. Blood PCR may be a useful tool for identifying subjects carrying C. pneumoniae in the vascular wall.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032751745&partnerID=8YFLogxK
U2 - 10.1086/315126
DO - 10.1086/315126
M3 - Article
C2 - 10558974
AN - SCOPUS:0032751745
VL - 180
SP - 2074
EP - 2076
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 6
ER -