Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis

Carlo Contini, Silva Seraceni, Massimiliano Castellazzi, Enrico Granieri, Enrico Fainardi

Research output: Contribution to journalArticlepeer-review

Abstract

Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalNeuroscience Research
Volume62
Issue number1
DOIs
Publication statusPublished - Sep 2008

Keywords

  • Cerebrospinal fluid
  • Chlamydophila pneumoniae
  • Culture
  • Multiple sclerosis
  • PBMC
  • PCR
  • RT-PCR

ASJC Scopus subject areas

  • Neuroscience(all)

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