Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis

Carlo Contini; Silva Seraceni; Massimiliano Castellazzi; Enrico Granieri; Enrico Fainardi

doi:10.1016/j.neures.2008.05.003

Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis

Carlo Contini, Silva Seraceni, Massimiliano Castellazzi, Enrico Granieri, Enrico Fainardi

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.

Original language	English
Pages (from-to)	58-61
Number of pages	4
Journal	Neuroscience Research
Volume	62
Issue number	1
DOIs	https://doi.org/10.1016/j.neures.2008.05.003
Publication status	Published - Sep 2008

Keywords

Cerebrospinal fluid
Chlamydophila pneumoniae
Culture
Multiple sclerosis
PBMC
PCR
RT-PCR

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neures.2008.05.003

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Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis. / Contini, Carlo; Seraceni, Silva; Castellazzi, Massimiliano; Granieri, Enrico; Fainardi, Enrico.

In: Neuroscience Research, Vol. 62, No. 1, 09.2008, p. 58-61.

Research output: Contribution to journal › Article › peer-review

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title = "Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis",

abstract = "Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.",

keywords = "Cerebrospinal fluid, Chlamydophila pneumoniae, Culture, Multiple sclerosis, PBMC, PCR, RT-PCR",

author = "Carlo Contini and Silva Seraceni and Massimiliano Castellazzi and Enrico Granieri and Enrico Fainardi",

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AU - Contini, Carlo

AU - Seraceni, Silva

AU - Castellazzi, Massimiliano

AU - Granieri, Enrico

AU - Fainardi, Enrico

PY - 2008/9

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N2 - Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.

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KW - Chlamydophila pneumoniae

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KW - Multiple sclerosis

KW - PBMC

KW - PCR

KW - RT-PCR

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Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis

Abstract

Keywords

ASJC Scopus subject areas

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