Abstract
The effect of in vitro treatment of human lymphocytes with rabbit cholesterol-rich serum (RCS) on the membrane microviscosity as well as on the β-adrenergic receptor density has been investigated. RCS treatment of cells resulted in a 30% decrease of receptor density without any effect on membrane microviscosity. A complete recovery was observed incubating the RCS cells either with the 'Active Lipids' (AL) or with heparin. The AL are a mixture of neutral lipids, phosphatidylcholine and phosphatidylethanolamine from hen egg yolk known to fluidify the cell membrane. The AL modified membrane microviscosity of control lymphocytes without altering their β-receptor number. These observations support the proposition that β-receptor density of human lymphocytes is not regulated by membrane microviscosity and suggest that probably low density lipoprotein-cholesterol complex is involved in such a regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 239-244 |
| Number of pages | 6 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 650 |
| Publication status | Published - 1992 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Cholesterol-rich rabbit serum modulates β-adrenergic receptor density of human lymphocytes : A possible role of LDL-cholesterol. / Pieri, C.; Moroni, F.; Recchioni, R.; Falasca, M.; Marcheselli, F.
In: Annals of the New York Academy of Sciences, Vol. 650, 1992, p. 239-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cholesterol-rich rabbit serum modulates β-adrenergic receptor density of human lymphocytes
T2 - A possible role of LDL-cholesterol
AU - Pieri, C.
AU - Moroni, F.
AU - Recchioni, R.
AU - Falasca, M.
AU - Marcheselli, F.
PY - 1992
Y1 - 1992
N2 - The effect of in vitro treatment of human lymphocytes with rabbit cholesterol-rich serum (RCS) on the membrane microviscosity as well as on the β-adrenergic receptor density has been investigated. RCS treatment of cells resulted in a 30% decrease of receptor density without any effect on membrane microviscosity. A complete recovery was observed incubating the RCS cells either with the 'Active Lipids' (AL) or with heparin. The AL are a mixture of neutral lipids, phosphatidylcholine and phosphatidylethanolamine from hen egg yolk known to fluidify the cell membrane. The AL modified membrane microviscosity of control lymphocytes without altering their β-receptor number. These observations support the proposition that β-receptor density of human lymphocytes is not regulated by membrane microviscosity and suggest that probably low density lipoprotein-cholesterol complex is involved in such a regulation.
AB - The effect of in vitro treatment of human lymphocytes with rabbit cholesterol-rich serum (RCS) on the membrane microviscosity as well as on the β-adrenergic receptor density has been investigated. RCS treatment of cells resulted in a 30% decrease of receptor density without any effect on membrane microviscosity. A complete recovery was observed incubating the RCS cells either with the 'Active Lipids' (AL) or with heparin. The AL are a mixture of neutral lipids, phosphatidylcholine and phosphatidylethanolamine from hen egg yolk known to fluidify the cell membrane. The AL modified membrane microviscosity of control lymphocytes without altering their β-receptor number. These observations support the proposition that β-receptor density of human lymphocytes is not regulated by membrane microviscosity and suggest that probably low density lipoprotein-cholesterol complex is involved in such a regulation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026686881&partnerID=8YFLogxK
M3 - Article
C2 - 1318659
AN - SCOPUS:0026686881
VL - 650
SP - 239
EP - 244
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -