Choline acetyltransferase of the common type immunoreactivity in the rat brain following different heroin treatments: A pilot study

Arianna Casini, Giorgio Vivacqua, Francesco E. Pontieri, Hiroshi Kimura, Jean P. Bellier, Loredana D'Este, Tindaro G. Renda

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies suggest that behavioral consequences of heroin treatment depend on the drug history of the animals and that cholinergic neurotransmission is involved in both behavioral and motor sensitization induced by heroin and other drugs of abuse. Immunohistochemistry, using a recently developed antiserum, specific for choline acetyl-transferase of the common type (cChAT), was applied to four different groups of rats, differing in drug regimens. Two groups of rats were submitted to the same schedule of heroin sensitization and then challenged for vehicle or heroin before sacrifice, obtaining two distinct groups, namely heroin-vehicle (HV) and heroin-heroin (HH). The same challenge was applied to another group of rats, previously submitted to a treatment with vehicle, obtaining other two groups, vehicle-vehicle (VV) and vehicle-heroin (VH), respectively. The number of cChAT-positive neurons is significantly increased (p0.05), increased in the nucleus accumbens core of heroin-sensitized rats (HV, HH). Instead, acute heroin treatment significantly increase (p

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalJournal of Chemical Neuroanatomy
Volume41
Issue number2
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Amygdala
  • Calretinin 31kDa
  • Cholinergic system
  • Drug sensitization
  • Nucleus accumbens

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Choline acetyltransferase of the common type immunoreactivity in the rat brain following different heroin treatments: A pilot study'. Together they form a unique fingerprint.

Cite this