Abstract
Proton magnetic resonance spectroscopy (1H-MRS) is largely exploited in clinical settings to non-invasively investigate chemical compounds in human tissues. Applications of 1H-MRS in oncology field are connected to the detection of abnormal levels of choline compounds in more active tumours, providing useful information for cancer diagnosis and treatment monitoring. Since benign lesions may also show presence of a choline peak, implementing absolute evaluation will help differentiating benign from malignant tumours. An external reference procedure was described to provide choline quantification in standard unit of measurements. Spectra were acquired on a 1.5T scanner using both phantoms and healthy volunteers with a PRESS sequence. The implemented quantification procedure used metabolite and noise measurements on the spectrum to remove large part of scanner settings contributing to metabolites of interest. A standard quantification was also used to compare performances ofthenoise-based method. Invitro quantification had accuracy and precision in the range (95-99)% and (5-13)%, respectively. When applied to invivo studies on healthy volunteers, the method provided very close values of choline concentration, more exactly (1.73±0.24) mmol/l. The method proposed can quantify the proper choline content in phantoms as well as in human structures, as brain. The method is ease of use, computational costless and it can be rapidly calibrated and implemented in any centre.
| Original language | English |
|---|---|
| Pages (from-to) | 677-683 |
| Number of pages | 7 |
| Journal | Physica Medica |
| Volume | 29 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 2013 |
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Keywords
- H-MRS
- Choline compounds
- In vivo evaluation
- Metabolite quantification
ASJC Scopus subject areas
- Biophysics
- Radiology Nuclear Medicine and imaging
- Physics and Astronomy(all)
Cite this
Choline-containing compounds quantification by 1H NMR spectroscopy using external reference and noise measurements. / Mazzetti, S.; Bracco, C.; Regge, D.; Caivano, R.; Russo, F.; Stasi, M.
In: Physica Medica, Vol. 29, No. 6, 11.2013, p. 677-683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Choline-containing compounds quantification by 1H NMR spectroscopy using external reference and noise measurements
AU - Mazzetti, S.
AU - Bracco, C.
AU - Regge, D.
AU - Caivano, R.
AU - Russo, F.
AU - Stasi, M.
PY - 2013/11
Y1 - 2013/11
N2 - Proton magnetic resonance spectroscopy (1H-MRS) is largely exploited in clinical settings to non-invasively investigate chemical compounds in human tissues. Applications of 1H-MRS in oncology field are connected to the detection of abnormal levels of choline compounds in more active tumours, providing useful information for cancer diagnosis and treatment monitoring. Since benign lesions may also show presence of a choline peak, implementing absolute evaluation will help differentiating benign from malignant tumours. An external reference procedure was described to provide choline quantification in standard unit of measurements. Spectra were acquired on a 1.5T scanner using both phantoms and healthy volunteers with a PRESS sequence. The implemented quantification procedure used metabolite and noise measurements on the spectrum to remove large part of scanner settings contributing to metabolites of interest. A standard quantification was also used to compare performances ofthenoise-based method. Invitro quantification had accuracy and precision in the range (95-99)% and (5-13)%, respectively. When applied to invivo studies on healthy volunteers, the method provided very close values of choline concentration, more exactly (1.73±0.24) mmol/l. The method proposed can quantify the proper choline content in phantoms as well as in human structures, as brain. The method is ease of use, computational costless and it can be rapidly calibrated and implemented in any centre.
AB - Proton magnetic resonance spectroscopy (1H-MRS) is largely exploited in clinical settings to non-invasively investigate chemical compounds in human tissues. Applications of 1H-MRS in oncology field are connected to the detection of abnormal levels of choline compounds in more active tumours, providing useful information for cancer diagnosis and treatment monitoring. Since benign lesions may also show presence of a choline peak, implementing absolute evaluation will help differentiating benign from malignant tumours. An external reference procedure was described to provide choline quantification in standard unit of measurements. Spectra were acquired on a 1.5T scanner using both phantoms and healthy volunteers with a PRESS sequence. The implemented quantification procedure used metabolite and noise measurements on the spectrum to remove large part of scanner settings contributing to metabolites of interest. A standard quantification was also used to compare performances ofthenoise-based method. Invitro quantification had accuracy and precision in the range (95-99)% and (5-13)%, respectively. When applied to invivo studies on healthy volunteers, the method provided very close values of choline concentration, more exactly (1.73±0.24) mmol/l. The method proposed can quantify the proper choline content in phantoms as well as in human structures, as brain. The method is ease of use, computational costless and it can be rapidly calibrated and implemented in any centre.
KW - H-MRS
KW - Choline compounds
KW - In vivo evaluation
KW - Metabolite quantification
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U2 - 10.1016/j.ejmp.2012.07.002
DO - 10.1016/j.ejmp.2012.07.002
M3 - Article
C2 - 22835985
AN - SCOPUS:84885951999
VL - 29
SP - 677
EP - 683
JO - Physica Medica
JF - Physica Medica
SN - 1120-1797
IS - 6
ER -