Choline metabolic profiling by magnetic resonance spectroscopy

Egidio Iorio, Alessandro Ricci, Maria Elena Pisanu, Marina Bagnoli, Franca Podo, Silvana Canevari

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The revised version of cancer hallmarks, depicting the biological properties acquired during tumor development and progression, includes the capability to modify or reprogram cellular metabolism. High-resolution multinuclear magnetic resonance spectroscopy (MRS) provides noninvasive means of monitoring metabolites that play a central role in several pathways, measuring the rates at which reactions within the pathways take place, and investigating how these rates are controlled in such a way that a metabolic precursor and cofactors are provided according to the demand. Here we describe comprehensive methods for assaying the activity of key enzymes involved in the phosphatidylcholine metabolic pathways responsible for phosphocholine accumulation in ovarian cancer cells using high-resolution 1H and 31P MRS analyses of cell lysates or cytosolic preparations.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages255-270
Number of pages16
Volume1049
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1049
ISSN (Print)10643745

Keywords

  • Choline kinase
  • Glycerophosphocholine phosphodiesterase
  • Magnetic resonance spectroscopy
  • Phosphatidylcholine metabolism
  • Phospholipases C and D specific for phosphatidylcholine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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