Chondrogenic capability of osteoarthritic chondrocytes from the trapeziometacarpal and hip joints

Arianna B. Lovati, Alessandra Colombini, Camilla Recordati, Cristina Ceriani, Luigi Zagra, Gianfranco Berzero, Matteo Moretti

Research output: Contribution to journalArticle

Abstract

Osteoarthritis is the most common degenerative disease of joints like the hip and the trapeziometacarpal joint (rhizarthrosis). In this in vitro study, we compared the chondrogenesis of chondrocytes derived from the trapezium and the femoral head cartilage of osteoarthritic patients to have a deeper insight on trapezium chondrocyte behavior as autologous cell source for the repair of cartilage lesions in rhizarthrosis. Chondrocytes collected from trapezium and femoral head articular cartilage were cultured in pellets and analyzed for chondrogenic differentiation, cell proliferation, glycosaminoglycan production, gene expression of chondrogenic and fibrous markers, histological and immunohistochemical analyses. Our results showed a higher cartilaginous matrix deposition and a lower fibrocartilaginous phenotype of the femoral chondrocytes with respect to the trapezium chondrocytes assessed by a higher absolute glycosaminoglycan and type II collagen production, thus demonstrating a superior chondrogenic potential of the femoral with respect to the trapezium chondrocytes. The differences in chondrogenic potential between trapezium and femoral head chondrocytes confirmed a lower regenerative capability in the trapezium than in the femoral head cartilage due to the different environment and loading acting on these joints that affects the metabolism of the resident cells. This could represent a limitation to apply the cell therapy for rhizoarthrosis.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalCell and Tissue Banking
Volume17
Issue number1
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Hip Joint
Cartilage
Chondrocytes
Thigh
Glycosaminoglycans
Collagen Type II
Cell proliferation
Collagen
Metabolism
Gene expression
Repair
Chondrogenesis
Joint Diseases
Articular Cartilage
Cell- and Tissue-Based Therapy
Osteoarthritis
Joints
Cell Proliferation
Phenotype
Gene Expression

Keywords

  • Cellular behaviour
  • Chondrocytes
  • Differentiation
  • Osteoarthritis
  • Trapezium

ASJC Scopus subject areas

  • Transplantation
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Chondrogenic capability of osteoarthritic chondrocytes from the trapeziometacarpal and hip joints. / Lovati, Arianna B.; Colombini, Alessandra; Recordati, Camilla; Ceriani, Cristina; Zagra, Luigi; Berzero, Gianfranco; Moretti, Matteo.

In: Cell and Tissue Banking, Vol. 17, No. 1, 01.03.2016, p. 171-177.

Research output: Contribution to journalArticle

Lovati, Arianna B. ; Colombini, Alessandra ; Recordati, Camilla ; Ceriani, Cristina ; Zagra, Luigi ; Berzero, Gianfranco ; Moretti, Matteo. / Chondrogenic capability of osteoarthritic chondrocytes from the trapeziometacarpal and hip joints. In: Cell and Tissue Banking. 2016 ; Vol. 17, No. 1. pp. 171-177.
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