Chondrogenic differentiation of murine and human mesenchymal stromal cells in a hyaluronic acid scaffold: Differences in gene expression and cell morphology

Gina Lisignoli, Sandra Cristino, Anna Piacentini, Nicoletta Zini, Danièle Noël, Christian Jorgensen, Andrea Facchini

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Chondrogenesis is a complex process characterized by a sequence of different steps that start with the condensation of the cells, followed by the expression of specific components, such as collagens and proteoglycans. We evaluated in vitro chondrogenic differentiation of C3H10T1/2 murine mesenchymal cells and compared them with human mesenchymal stromal cells (h-MSCs) in a hyaluronic acid scaffold. We analyzed (from day 0 to day 28) cellular morphology, proliferation, and chondrogenic/osteogenic gene expression at different time points. Our data demonstrate that, during chondrogenic differentiation, murine cells proliferate both in the absence and presence of TGFB, while h-MSCs require the presence of this activating factor. Murine cells, even if viable, differentiate on hyaluronan scaffold, maintain a fibroblastic morphology, and form a capsule outside the scaffold. At the mRNA level, murine cells showed a decrease in collagen type I combined with a significant increase in collagen type II (from day 0), and aggrecan (on day 28), as found for h-MSCs. Immunohistochemical data confirmed that chondrogenic differentiation of murine cells, induced by TGFβ, occurred only in some restricted areas inside the scaffold that were positive to collagen type II, but did not show a cartilage-like tissue structure, as we had found using h-MSCs. These data demonstrate that C3H10T1/2 murine cells, widely used as a chondrogenic model, show a different sequence of chondrogenic events in hyaluronic acid scaffold, compared with primary h-MSCs.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume77
Issue number3
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Hyaluronic acid
Hyaluronic Acid
Mesenchymal Stromal Cells
Collagen
Gene expression
Scaffolds
Gene Expression
Collagen Type II
Cell Differentiation
Forms (concrete)
Cartilage
Scaffolds (biology)
Aggrecans
Chondrogenesis
Condensation
Proteoglycans
Collagen Type I
Capsules
Tissue
Cell Proliferation

Keywords

  • Cell morphology
  • Cell proliferation
  • Chondrogenesis
  • Chondrogenic markers
  • Osteogenic markers

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Chondrogenic differentiation of murine and human mesenchymal stromal cells in a hyaluronic acid scaffold : Differences in gene expression and cell morphology. / Lisignoli, Gina; Cristino, Sandra; Piacentini, Anna; Zini, Nicoletta; Noël, Danièle; Jorgensen, Christian; Facchini, Andrea.

In: Journal of Biomedical Materials Research - Part A, Vol. 77, No. 3, 01.06.2006, p. 497-506.

Research output: Contribution to journalArticle

Lisignoli, Gina ; Cristino, Sandra ; Piacentini, Anna ; Zini, Nicoletta ; Noël, Danièle ; Jorgensen, Christian ; Facchini, Andrea. / Chondrogenic differentiation of murine and human mesenchymal stromal cells in a hyaluronic acid scaffold : Differences in gene expression and cell morphology. In: Journal of Biomedical Materials Research - Part A. 2006 ; Vol. 77, No. 3. pp. 497-506.
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