Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation

Riccardo Albertini, Pilar Ramos, Andreas Giessauf, Alberto Passi, Giancarlo De Luca, Hermann Esterbauer

Research output: Contribution to journalArticle

Abstract

Chondroitin 4-sulphate (C4S), a basic component of the extracellular matrix of the artery wall, inhibited copper-induced low density lipoprotein (LDL) oxidation by prolonging the log time and reducing the rate of propagation, LDL oxidation was assessed by monitoring conjugated dienes and low level chemiluminescence. A possible initial key reaction in LDL oxidation, the reduction of copper(II) to copper(I) by LDL, was decreased in the presence of C4S. Moreover, C4S protected tryptophan residues of Apo-B-100 in the early stage of LDL oxidation and during the subsequent propagation phase. The presence of the sulphate group in position 4 of N-acetylgalactosaminyl residues of C4S is crucial for protective activity. In fact, the structurally related chondroitin 6-sulphate, also present in tissues, had no effect on LDL oxidation. These data suggest that C4S may contribute to protect LDL against oxidation in arterial intima.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalFEBS Letters
Volume403
Issue number2
DOIs
Publication statusPublished - Feb 17 1997

Fingerprint

Chondroitin Sulfates
LDL Lipoproteins
Oxidation
Copper
Tunica Intima
Apolipoprotein B-100
Chemiluminescence
Luminescence
Tryptophan
Sulfates
Oxidation-Reduction
Extracellular Matrix
Arteries
Tissue
Monitoring

Keywords

  • Chondroitin sulfate
  • Copper oxidation
  • Glycosaminoglycan
  • Low density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation. / Albertini, Riccardo; Ramos, Pilar; Giessauf, Andreas; Passi, Alberto; De Luca, Giancarlo; Esterbauer, Hermann.

In: FEBS Letters, Vol. 403, No. 2, 17.02.1997, p. 154-158.

Research output: Contribution to journalArticle

Albertini, R, Ramos, P, Giessauf, A, Passi, A, De Luca, G & Esterbauer, H 1997, 'Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation', FEBS Letters, vol. 403, no. 2, pp. 154-158. https://doi.org/10.1016/S0014-5793(97)00043-4
Albertini, Riccardo ; Ramos, Pilar ; Giessauf, Andreas ; Passi, Alberto ; De Luca, Giancarlo ; Esterbauer, Hermann. / Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation. In: FEBS Letters. 1997 ; Vol. 403, No. 2. pp. 154-158.
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AU - Esterbauer, Hermann

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