Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation

Riccardo Albertini, Pilar Ramos, Andreas Giessauf, Alberto Passi, Giancarlo De Luca, Hermann Esterbauer

Research output: Contribution to journalArticle

Abstract

Chondroitin 4-sulphate (C4S), a basic component of the extracellular matrix of the artery wall, inhibited copper-induced low density lipoprotein (LDL) oxidation by prolonging the log time and reducing the rate of propagation, LDL oxidation was assessed by monitoring conjugated dienes and low level chemiluminescence. A possible initial key reaction in LDL oxidation, the reduction of copper(II) to copper(I) by LDL, was decreased in the presence of C4S. Moreover, C4S protected tryptophan residues of Apo-B-100 in the early stage of LDL oxidation and during the subsequent propagation phase. The presence of the sulphate group in position 4 of N-acetylgalactosaminyl residues of C4S is crucial for protective activity. In fact, the structurally related chondroitin 6-sulphate, also present in tissues, had no effect on LDL oxidation. These data suggest that C4S may contribute to protect LDL against oxidation in arterial intima.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalFEBS Letters
Volume403
Issue number2
DOIs
Publication statusPublished - Feb 17 1997

Keywords

  • Chondroitin sulfate
  • Copper oxidation
  • Glycosaminoglycan
  • Low density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation'. Together they form a unique fingerprint.

  • Cite this