Chromatin organisation and nuclear architecture in growing mouse oocytes

Maurizio Zuccotti, Silvia Garagna, Valeria Merico, Manuela Monti, Carlo Alberto Redi

Research output: Contribution to journalArticle

Abstract

Although the female gamete is blocked at the dictyate stage of the first meiotic prophase during the whole folliculogenesis, many important epigenetic changes occur to organise the genome to attend early embryonic development. In this paper, we will describe the results of a number of studies aimed to improve our understanding of the nuclear organization of the mouse oocyte during folliculogenesis. Using silver methods that stain NOR, centromeres and heterochromatin, as well as, the use of specific antibodies for the demonstration of centromeres, we have described the changes to the chromatin organisation and to the spatial localisation of chromocenters and centromeres during oocyte growth; these changes have been correlated to the developmental competence of the resulting antral and metaphase II (MII) oocyte.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume234
Issue number1-2
DOIs
Publication statusPublished - Apr 29 2005

Keywords

  • Chromatin organisation
  • Folliculogenesis
  • Oocyte

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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