Abstract
Time relationships of sperm chromatin dispersion and sperm nucleoprotein replacement have been studied in vivo, by an in situ cytochemical approach. We used the Feulgen reaction to reveal DNA, which allow us to record both processes simultaneously, on the basis of the return after fertilization to haploid Feulgen values after sperm nucleoprotein replacement with somatic histones. We have shown that sperm nucleoprotein replacement occurs at around anaphase II, whereas sperm chromatin dispersion is massive between the anaphase and telophase II oocyte phases. The morphological pattern of sperm chromatin dispersion supports the idea that the process involves the whole sperm chromatin mass simultaneously, with the region located between the implantation fossa and the postacrosomial region the last to swell.
| Original language | English |
|---|---|
| Pages (from-to) | 187-193 |
| Number of pages | 7 |
| Journal | Journal of Experimental Zoology |
| Volume | 246 |
| Issue number | 2 |
| Publication status | Published - May 1988 |
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ASJC Scopus subject areas
- Animal Science and Zoology
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Chromatin topology during the transformation of the mouse sperm nucleus into pronucleus in vivo. / Garagna, S.; Redi, C. A.
In: Journal of Experimental Zoology, Vol. 246, No. 2, 05.1988, p. 187-193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chromatin topology during the transformation of the mouse sperm nucleus into pronucleus in vivo.
AU - Garagna, S.
AU - Redi, C. A.
PY - 1988/5
Y1 - 1988/5
N2 - Time relationships of sperm chromatin dispersion and sperm nucleoprotein replacement have been studied in vivo, by an in situ cytochemical approach. We used the Feulgen reaction to reveal DNA, which allow us to record both processes simultaneously, on the basis of the return after fertilization to haploid Feulgen values after sperm nucleoprotein replacement with somatic histones. We have shown that sperm nucleoprotein replacement occurs at around anaphase II, whereas sperm chromatin dispersion is massive between the anaphase and telophase II oocyte phases. The morphological pattern of sperm chromatin dispersion supports the idea that the process involves the whole sperm chromatin mass simultaneously, with the region located between the implantation fossa and the postacrosomial region the last to swell.
AB - Time relationships of sperm chromatin dispersion and sperm nucleoprotein replacement have been studied in vivo, by an in situ cytochemical approach. We used the Feulgen reaction to reveal DNA, which allow us to record both processes simultaneously, on the basis of the return after fertilization to haploid Feulgen values after sperm nucleoprotein replacement with somatic histones. We have shown that sperm nucleoprotein replacement occurs at around anaphase II, whereas sperm chromatin dispersion is massive between the anaphase and telophase II oocyte phases. The morphological pattern of sperm chromatin dispersion supports the idea that the process involves the whole sperm chromatin mass simultaneously, with the region located between the implantation fossa and the postacrosomial region the last to swell.
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M3 - Article
C2 - 3392516
AN - SCOPUS:0024006647
VL - 246
SP - 187
EP - 193
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
SN - 0022-104X
IS - 2
ER -