Chromosome 11q23.1 is an unstable region in B-cell tumor cell lines

Giulia Poretti, Ivo Kwee, Barbara Bernasconi, Paola M V Rancoita, Andrea Rinaldi, Carlo Capella, Emanuele Zucca, Antonino Neri, Maria Grazia Tibiletti, Francesco Bertoni

Research output: Contribution to journalArticle

Abstract

Chromosome 11q23 region is a frequent target of chromosome aberrations in B-cell lymphoid tumors. Here, we present the cytogenetic and molecular characterization of an amplification affecting 11q23.1 in four cell lines derived from B-cell lymphoid tumors. A minimal common region of amplification of 330. kb was identified in three cell lines using Affymetrix Human Mapping 250K arrays. When analyzed with three BAC clones, the amplifications appeared different at cytogenetic level in each cell line. Possibly affected transcripts were evaluated using tiling arrays, and validated by real time PCR. Since no effect of the amplification at the local transcription level was observed, it is possible that 11q23 amplification might mainly represent the effect of unstable chromosomal region.

Original languageEnglish
Pages (from-to)808-813
Number of pages6
JournalLeukemia Research
Volume35
Issue number6
DOIs
Publication statusPublished - Jun 2011

Keywords

  • FISH, array
  • Lymphoma
  • POUAF1
  • PPP2R1B
  • SIK2
  • Tiling
  • Transcriptome
  • Translocation

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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